BMRB Entry 11139

Name: Solution structure of the 1st KH type I domain from human Vigilin
Published: 2011-04-01

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PDB ID: 2cte
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11139
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 1st KH type I domain from human Vigilin

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 1st KH type I domain from human Vigilin" .

Assembly members:

Assembly members:
KH domain, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050131-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KH domain: GSSGSSGDIVARLQTQASAT VAIPKEHHRFVIGKNGEKLQ DLELKTATKIQIPRPDDPSN QIKITGTKEGIEKARHEVLL ISAEQDKRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	390
15N chemical shifts	89
1H chemical shifts	633

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KH domain	1

Entities:

Entity 1, KH domain 94 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

ILE

VAL

2

ALA

ARG

LEU

GLN

THR

GLN

ALA

SER

ALA

THR

3

VAL

ALA

ILE

PRO

LYS

GLU

HIS

ARG

PHE

4

VAL

ILE

GLY

LYS

ASN

GLY

GLU

LYS

LEU

GLN

5

ASP

LEU

GLU

LEU

LYS

THR

ALA

THR

LYS

ILE

6

GLN

ILE

PRO

ARG

PRO

ASP

PRO

SER

ASN

7

GLN

ILE

LYS

ILE

THR

GLY

THR

LYS

GLU

GLY

8

ILE

GLU

LYS

ALA

ARG

HIS

GLU

VAL

LEU

9

ILE

SER

ALA

GLU

GLN

ASP

LYS

ARG

SER

GLY

10

PRO

SER

GLY

Samples:

sample_1: KH domain, [U-13C; U-15N], 1.05 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, obayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz