BMRB Entry 11132

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PDB ID: 2cto
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11132
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the HMG box like domain from human hypothetical protein FLJ14904

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.. "Solution structure of the HMG box like domain from human hypothetical protein FLJ14904" .

Assembly members:

Assembly members:
HMG box like domain, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050125-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HMG box like domain: GSSGSSGMPNRKASRNAYYF FVQEKIPELRRRGLPVARVA DAIPYCSSDWALLREEEKEK YAEMAREWRAAQGKDPGPSE KQKPVFTSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	383
15N chemical shifts	80
1H chemical shifts	592

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HMG box like domain	1

Entities:

Entity 1, HMG box like domain 93 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

PRO

ASN

2

ARG

LYS

ALA

SER

ARG

ASN

ALA

TYR

PHE

3

PHE

VAL

GLN

GLU

LYS

ILE

PRO

GLU

LEU

ARG

4

ARG

GLY

LEU

PRO

VAL

ALA

ARG

VAL

ALA

5

ASP

ALA

ILE

PRO

TYR

CYS

SER

ASP

TRP

6

ALA

LEU

ARG

GLU

LYS

GLU

LYS

7

TYR

ALA

GLU

MET

ALA

ARG

GLU

TRP

ARG

ALA

8

ALA

GLN

GLY

LYS

ASP

PRO

GLY

PRO

SER

GLU

9

LYS

GLN

LYS

PRO

VAL

PHE

THR

SER

GLY

PRO

10

SER

GLY

Samples:

sample_1: HMG box like domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CTO
DBJ	BAB55385 BAE01036 BAJ20777
GB	AAH28595 AAY82463 ADQ32386 AIC52639 EAW90780
REF	NP_001247890 NP_116247 XP_002809887 XP_003785786 XP_003893548
SP	Q4R786 Q96JY0
AlphaFold	Q4R786 Q96JY0