BMRB Entry 11130

Title:
Solution structure of two zf-C2H2 domains from human Zinc finger protein 512
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of two zf-C2H2 domains from human Zinc finger protein 512"  .

Assembly members:

Assembly members:
zf-C2H2 domains, polymer, 96 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041018-04

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts386
15N chemical shifts89
1H chemical shifts610

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zf-C2H2 domains1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, zf-C2H2 domains 96 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGILEARG
2   LYSGLUPROPROVALTYRALAALAGLYSER
3   LEUGLUGLUGLNTRPTYRLEUGLUILEVAL
4   ASPLYSGLYSERVALSERCYSPROTHRCYS
5   GLNALAVALGLYARGLYSTHRILEGLUGLY
6   LEULYSLYSHISMETGLUASNCYSLYSGLN
7   GLUMETPHETHRCYSHISHISCYSGLYLYS
8   GLNLEUARGSERLEUALAGLYMETLYSTYR
9   HISVALMETALAASNHISASNSERLEUPRO
10   SERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zf-C2H2 domains, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; ZnCl2 0.25 mM; IDA 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB EHH21997 EHH55467
REF XP_007191412 XP_008831438 XP_010989556 XP_014418967

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks