BMRB Entry 11119

Title:
The solution structure of the first fibronectin type III domain of human Neogenin
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the first fibronectin type III domain of human Neogenin"  .

Assembly members:

Assembly members:
fn3 domain, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041213-02

Data sets:
Data typeCount
13C chemical shifts486
15N chemical shifts114
1H chemical shifts752

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fn3 domain1

Entities:

Entity 1, fn3 domain 120 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUHISALA
2   PROALATHRTHRGLYPROLEUPROSERALA
3   PROARGASPVALVALALASERLEUVALSER
4   THRARGPHEILELYSLEUTHRTRPARGTHR
5   PROALASERASPPROHISGLYASPASNLEU
6   THRTYRSERVALPHETYRTHRLYSGLUGLY
7   ILEALAARGGLUARGVALGLUASNTHRSER
8   HISPROGLYGLUMETGLNVALTHRILEGLN
9   ASNLEUMETPROALATHRVALTYRILEPHE
10   ARGVALMETALAGLNASNLYSHISGLYSER
11   GLYGLUSERSERALAPROLEUARGVALGLU
12   THRGLNPROGLUSERGLYPROSERSERGLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.9 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks