BMRB Entry 11077

Title:
Solution structure of the E. coli ribosome hibernation promoting factor HPF
Deposition date:
2009-08-13
Original release date:
2009-10-14
Authors:
Sato, Akiko; Mishima, Masaki
Citation:

Citation: Sato, Akiko; Watanabe, Takumi; Maki, Yasushi; Ueta, Masami; Yoshida, Hideji; Ito, Yutaka; Wada, Akira; Mishima, Masaki. "Solution structure of the E. coli ribosome hibernation promoting factor HPF: Implications for the relationship between structure and function."  Biochem. Biophys. Res. Commun. 389, 580-585 (2009).
PubMed: 19747895

Assembly members:

Assembly members:
HPF, polymer, 95 residues, 10767.349 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE9

Data sets:
Data typeCount
13C chemical shifts312
15N chemical shifts94
1H chemical shifts641

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HPF1

Entities:

Entity 1, HPF 95 residues - 10767.349 Da.

The residue Met-1 is derived from the expression vector. Native protein sequence starts second Gln as Gln-1.

1   METGLNLEUASNILETHRGLYASNASNVAL
2   GLUILETHRGLUALALEUARGGLUPHEVAL
3   THRALALYSPHEALALYSLEUGLUGLNTYR
4   PHEASPARGILEASNGLNVALTYRVALVAL
5   LEULYSVALGLULYSVALTHRHISTHRSER
6   ASPALATHRLEUHISVALASNGLYGLYGLU
7   ILEHISALASERALAGLUGLYGLNASPMET
8   TYRALAALAILEASPGLYLEUILEASPLYS
9   LEUALAARGGLNLEUTHRLYSHISLYSASP
10   LYSLEULYSGLNHIS

Related Database Links:

PDB 2RQL 3V26 3V28
DBJ BAA02316 BAB37505 BAE77247 BAG79011 BAI27481
EMBL CAA81618 CAP77663 CAQ33536 CAR00165 CAR04813
GB AAA58005 AAB60164 AAC76235 AAG58337 AAN44709
REF NP_312109 NP_417670 NP_709002 WP_001176593 WP_001176595
SP P0AFX0 P0AFX1 P0AFX2 P0AFX3
TPD FAA00071
AlphaFold P0AFX0 P0AFX1 P0AFX2 P0AFX3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks