BMRB Entry 11038

Assembly:

Entities:

Entity 1, The DNA binding domain of RTBP1 122 residues - 13862.927 Da.

First G and S and "VDSSGRIVTL" in C-terminal were from cloining artifacts.

Samples:

sample_1: The DNA binding domai of RTBP1, [U-99% 15N], 1 mM; Potassium phosphate 50 mM; NaCl 100 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_2: The DNA binding domai of RTBP1, [U-100% 13C], 1 mM; Potassium phosphate 50 mM; NaCl 100 mM; NaN3 1 mM; D2O 100%

sample_3: The DNA binding domai of RTBP1, [U-99% 13C; U-99% 15N], 1 mM; Potassium phosphate 50 mM; NaCl 100 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 Pa; temperature: 303 K

Experiments:

Software:

CYANA v2.2.5, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

CYANA, Bruker Biospin - processing

CYANA, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CYANA, Goddard - chemical shift assignment, peak picking

CYANA, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

• Bruker DRX 500 MHz
• Bruker DRX 900 MHz