BMRB Entry 11030

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PDB ID: 2rno
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR11030
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the N-terminal SAP Domain of SUMO E3 Ligases from Oryza sativa

Deposition date:

2008-01-29

Original release date:

2008-11-11

Authors:

Suzuki, Rintaro; Shindo, Heisaburo; Tase, Akira; Yamazaki, Toshimasa

Citation:

Citation: Suzuki, Rintaro; Shindo, Heisaburo; Tase, Akira; Kikuchi, Yoshiko; Shimizu, Mitsuhiro; Yamazaki, Toshimasa. "Solution Structures and DNA Binding Properties of the N-terminal SAP Domains of SUMO E3 Ligases from Saccharomyces cerevisiae and Oryza sativa" Proteins 75, 336-347 (2008).
PubMed: 18831036

Assembly members:

Assembly members:
Siz1 1-105, polymer, 110 residues, 12388.231 Da.

Natural source:

Natural source: Common Name: Oryza sativa Taxonomy ID: 39947 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oryza sativa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Siz1 1-105: GSHMASADLVSSCKDKLAYF RIKELKDILNQLGLPKQGKK QDLIDRVLALLTDEQGQRHH GWGRKNSLTKEAVAKIVDDT YRKMQIQCAPDLATRSHSGS DFSFRPIEEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	927
15N chemical shifts	244
1H chemical shifts	1468

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Siz1 1-105	1

Entities:

Entity 1, Siz1 1-105 110 residues - 12388.231 Da.

Residues (-5)-1 represent a non-native sequence from affinity tag.

1	GLY	SER	HIS	MET	ALA	SER	ALA	ASP	LEU	VAL
2	SER	SER	CYS	LYS	ASP	LYS	LEU	ALA	TYR	PHE
3	ARG	ILE	LYS	GLU	LEU	LYS	ASP	ILE	LEU	ASN
4	GLN	LEU	GLY	LEU	PRO	LYS	GLN	GLY	LYS	LYS
5	GLN	ASP	LEU	ILE	ASP	ARG	VAL	LEU	ALA	LEU
6	LEU	THR	ASP	GLU	GLN	GLY	GLN	ARG	HIS	HIS
7	GLY	TRP	GLY	ARG	LYS	ASN	SER	LEU	THR	LYS
8	GLU	ALA	VAL	ALA	LYS	ILE	VAL	ASP	ASP	THR
9	TYR	ARG	LYS	MET	GLN	ILE	GLN	CYS	ALA	PRO
10	ASP	LEU	ALA	THR	ARG	SER	HIS	SER	GLY	SER
11	ASP	PHE	SER	PHE	ARG	PRO	ILE	GLU	GLU	ALA

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	13C_15N_in_10%_D2O	isotropic	pH_6.1
3D 1H-15N NOESY	13C_15N_in_10%_D2O	isotropic	pH_6.1
3D HNCO	13C_15N_in_10%_D2O	isotropic	pH_6.1
3D HNCA	13C_15N_in_10%_D2O	isotropic	pH_6.1
3D CBCA(CO)NH	13C_15N_in_10%_D2O	isotropic	pH_6.1
3D 13C-edited 1H-15N NOESY	13C_15N_in_10%_D2O	isotropic	pH_6.1
2D 1H-13C HSQC	13C_15N_in_100%_D2O	isotropic	pH_6.1
3D HCCH-TOCSY	13C_15N_in_100%_D2O	isotropic	pH_6.1
4D 1H-13C NOESY	13C_15N_in_100%_D2O	isotropic	pH_6.1
3D HCABGCO	13C_15N_in_100%_D2O	isotropic	pH_6.1

PDB	2RNO
DBJ	BAF16431 BAG97182 BAS92058
GB	AAT39226 EEC78434 EEE62174
REF	NP_001054517
SP	Q6L4L4
AlphaFold	Q6L4L4

BMRB Entry 11030

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	HIS	MET	ALA	SER	ALA	ASP	LEU	VAL
2	SER	SER	CYS	LYS	ASP	LYS	LEU	ALA	TYR	PHE
3	ARG	ILE	LYS	GLU	LEU	LYS	ASP	ILE	LEU	ASN
4	GLN	LEU	GLY	LEU	PRO	LYS	GLN	GLY	LYS	LYS
5	GLN	ASP	LEU	ILE	ASP	ARG	VAL	LEU	ALA	LEU
6	LEU	THR	ASP	GLU	GLN	GLY	GLN	ARG	HIS	HIS
7	GLY	TRP	GLY	ARG	LYS	ASN	SER	LEU	THR	LYS
8	GLU	ALA	VAL	ALA	LYS	ILE	VAL	ASP	ASP	THR
9	TYR	ARG	LYS	MET	GLN	ILE	GLN	CYS	ALA	PRO
10	ASP	LEU	ALA	THR	ARG	SER	HIS	SER	GLY	SER
11	ASP	PHE	SER	PHE	ARG	PRO	ILE	GLU	GLU	ALA

1	GLY	SER	HIS	MET	ALA	SER	ALA	ASP	LEU	VAL
2	SER	SER	CYS	LYS	ASP	LYS	LEU	ALA	TYR	PHE
3	ARG	ILE	LYS	GLU	LEU	LYS	ASP	ILE	LEU	ASN
4	GLN	LEU	GLY	LEU	PRO	LYS	GLN	GLY	LYS	LYS
5	GLN	ASP	LEU	ILE	ASP	ARG	VAL	LEU	ALA	LEU
6	LEU	THR	ASP	GLU	GLN	GLY	GLN	ARG	HIS	HIS
7	GLY	TRP	GLY	ARG	LYS	ASN	SER	LEU	THR	LYS
8	GLU	ALA	VAL	ALA	LYS	ILE	VAL	ASP	ASP	THR
9	TYR	ARG	LYS	MET	GLN	ILE	GLN	CYS	ALA	PRO
10	ASP	LEU	ALA	THR	ARG	SER	HIS	SER	GLY	SER
11	ASP	PHE	SER	PHE	ARG	PRO	ILE	GLU	GLU	ALA

1	GLY	SER	HIS	MET	ALA	SER	ALA	ASP	LEU	VAL
2	SER	SER	CYS	LYS	ASP	LYS	LEU	ALA	TYR	PHE
3	ARG	ILE	LYS	GLU	LEU	LYS	ASP	ILE	LEU	ASN
4	GLN	LEU	GLY	LEU	PRO	LYS	GLN	GLY	LYS	LYS
5	GLN	ASP	LEU	ILE	ASP	ARG	VAL	LEU	ALA	LEU
6	LEU	THR	ASP	GLU	GLN	GLY	GLN	ARG	HIS	HIS
7	GLY	TRP	GLY	ARG	LYS	ASN	SER	LEU	THR	LYS
8	GLU	ALA	VAL	ALA	LYS	ILE	VAL	ASP	ASP	THR
9	TYR	ARG	LYS	MET	GLN	ILE	GLN	CYS	ALA	PRO
10	ASP	LEU	ALA	THR	ARG	SER	HIS	SER	GLY	SER
11	ASP	PHE	SER	PHE	ARG	PRO	ILE	GLU	GLU	ALA