BMRB Entry 10327

Name: Solution structure of the filamin domain from human tripartite motif protein 45
Published: 2010-04-07

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PDB ID: 2ds4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10327
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the filamin domain from human tripartite motif protein 45

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the filamin domain from human tripartite motif protein 45" .

Assembly members:

Assembly members:
Filamin domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060227-22

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGEVDPAKCVLQGED LHRAREKQTASFTLLCKDAA GEIMGRGGDNVQVAVVPKDK KDSPVRTMVQDNKDGTYYIS YTPKEPGVYTVWVCIKEQHV QGSPFTVTVRRKH

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	488
15N chemical shifts	113
1H chemical shifts	765

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 113 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

VAL

ASP

2

PRO

ALA

LYS

CYS

VAL

LEU

GLN

GLY

GLU

ASP

3

LEU

HIS

ARG

ALA

ARG

GLU

LYS

GLN

THR

ALA

4

SER

PHE

THR

LEU

CYS

LYS

ASP

ALA

5

GLY

GLU

ILE

MET

GLY

ARG

GLY

ASP

ASN

6

VAL

GLN

VAL

ALA

VAL

PRO

LYS

ASP

LYS

7

LYS

ASP

SER

PRO

VAL

ARG

THR

MET

VAL

GLN

8

ASP

ASN

LYS

ASP

GLY

THR

TYR

ILE

SER

9

TYR

THR

PRO

LYS

GLU

PRO

GLY

VAL

TYR

THR

10

VAL

TRP

VAL

CYS

ILE

LYS

GLU

GLN

HIS

VAL

11

GLN

GLY

SER

PRO

PHE

THR

VAL

THR

VAL

ARG

12

ARG

LYS

HIS

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DS4
DBJ	BAB14484 BAD96621
GB	AAH34943 AAT76864 ABZ92546 EAW56668
REF	NP_001139107 XP_003806334 XP_004026476 XP_008950245