BMRB Entry 10323

Name: Solution structure of the 13th filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2dj4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10323
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 13th filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 13th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060123-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGVVDPSKVKIAGPG LGSGVRARVLQSFTVDSSKA GLAPLEVRVLGPRGLVEPVN VVDNGDGTHTVTYTPSQEGP YMVSVKYADEEIPRSPFKVK VLPTYDAS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	446
15N chemical shifts	96
1H chemical shifts	699

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 108 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

ASP

2

PRO

SER

LYS

VAL

LYS

ILE

ALA

GLY

PRO

GLY

3

LEU

GLY

SER

GLY

VAL

ARG

ALA

ARG

VAL

LEU

4

GLN

SER

PHE

THR

VAL

ASP

SER

LYS

ALA

5

GLY

LEU

ALA

PRO

LEU

GLU

VAL

ARG

VAL

LEU

6

GLY

PRO

ARG

GLY

LEU

VAL

GLU

PRO

VAL

ASN

7

VAL

ASP

ASN

GLY

ASP

GLY

THR

HIS

THR

8

VAL

THR

TYR

THR

PRO

SER

GLN

GLU

GLY

PRO

9

TYR

MET

VAL

SER

VAL

LYS

TYR

ALA

ASP

GLU

10

GLU

ILE

PRO

ARG

SER

PRO

PHE

LYS

VAL

LYS

11

VAL

LEU

PRO

THR

TYR

ASP

ALA

SER

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060324, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz