BMRB Entry 10288

Title:
Solution structure of the homeobox domain of Hepatocyte nuclear factor 1-beta (HNF-1beta)
Deposition date:
2008-12-17
Original release date:
2009-12-17
Authors:
Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of Hepatocyte nuclear factor 1-beta (HNF-1beta)"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P050404-17

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts99
1H chemical shifts623

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 102 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGASNARG
2   PHELYSTRPGLYPROALASERGLNGLNILE
3   LEUTYRGLNALATYRASPARGGLNLYSASN
4   PROSERLYSGLUGLUARGGLUALALEUVAL
5   GLUGLUCYSASNARGALAGLUCYSLEUGLN
6   ARGGLYVALSERPROSERLYSALAHISGLY
7   LEUGLYSERASNLEUVALTHRGLUVALARG
8   VALTYRASNTRPPHEALAASNARGARGLYS
9   GLUGLUALAPHEARGGLNLYSLEUALAMET
10   ASPALATYRSERSERASNSERGLYPROSER
11   SERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA77718 BAA77719 BAA77720 BAA77721 BAB23604
EMBL CAA39358 CAA39886 CAA41652 CAA49356 CAB59223
GB AAC63388 AAH17714 AAH25189 AAH81826 AAI29148
REF NP_000449 NP_001124797 NP_001159395 NP_001179784 NP_001181477
SP A1L1N5 P23899 P27889 P35680 Q03365
TPG DAA19061 DAA19062
AlphaFold P35680 A1L1N5 P23899 P27889 Q03365

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks