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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10229
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Tochio, N.; Tomizawa, T.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc finger (region 385-413) of human Zinc
finger protein 268" .
Assembly members:
zf-C2H2, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Vector: P061218-05
Entity Sequences (FASTA):
zf-C2H2: GSSGSSGQKPYVCNECGKAF
GLKSQLIIHERIHTGESGPS
SG
Data type | Count |
13C chemical shifts | 148 |
15N chemical shifts | 34 |
1H chemical shifts | 227 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | zf-C2H2 | 1 |
2 | ZINC ION | 2 |
Entity 1, zf-C2H2 42 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | LYS | PRO | ||||
2 | TYR | VAL | CYS | ASN | GLU | CYS | GLY | LYS | ALA | PHE | ||||
3 | GLY | LEU | LYS | SER | GLN | LEU | ILE | ILE | HIS | GLU | ||||
4 | ARG | ILE | HIS | THR | GLY | GLU | SER | GLY | PRO | SER | ||||
5 | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
1 | ZN |
sample_1: zf-C2H2, [U-13C; U-15N], 1.0 ± 0.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 0.05 mM; IDA 1 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
PDB |
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