BMRB Entry 10181

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PDB ID: 2em9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10181
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C2H2 type zinc finger (region 367-399) of human Zinc finger protein 224

Deposition date:

2008-02-06

Original release date:

2009-02-26

Authors:

Tomizawa, T.; Tochio, N.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Tomizawa, T.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc finger (region 367-399) of human Zinc finger protein 224" .

Assembly members:

Assembly members:
zf-C2H2, polymer, 46 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P061225-23

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zf-C2H2: GSSGSSGTGEKPYNCKECGK SFRWASCLLKHQRVHSGEKP SGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	155
15N chemical shifts	35
1H chemical shifts	234

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zf-C2H2	1
2	ZINC ION	2

Entities:

Entity 1, zf-C2H2 46 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

GLY

GLU

2

LYS

PRO

TYR

ASN

CYS

LYS

GLU

CYS

GLY

LYS

3

SER

PHE

ARG

TRP

ALA

SER

CYS

LEU

LYS

4

HIS

GLN

ARG

VAL

HIS

SER

GLY

GLU

LYS

PRO

5

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zf-C2H2, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 0.05 mM; IDA 1 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz