BMRB Entry 10163

Name: Solution structure of the 24th filamin domain from human Filamin-B
Published: 2009-02-20

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PDB ID: 2eed
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10163
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 24th filamin domain from human Filamin-B

Deposition date:

2007-12-28

Original release date:

2009-02-20

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 24th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
filamin domain, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060613-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
filamin domain: GSSGSSGSSDASKVTSKGAG LSKAFVGQKSSFLVDCSKAG SNMLLIGVHGPTTPCEEVSM KHVGNQQYNVTYVVKERGDY VLAVKWGEEHIPGSPFHVTV P

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	416
15N chemical shifts	97
1H chemical shifts	647

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-B	1

Entities:

Entity 1, Filamin-B 101 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

ASP

2

ALA

SER

LYS

VAL

THR

SER

LYS

GLY

ALA

GLY

3

LEU

SER

LYS

ALA

PHE

VAL

GLY

GLN

LYS

SER

4

SER

PHE

LEU

VAL

ASP

CYS

SER

LYS

ALA

GLY

5

SER

ASN

MET

LEU

ILE

GLY

VAL

HIS

GLY

6

PRO

THR

PRO

CYS

GLU

VAL

SER

MET

7

LYS

HIS

VAL

GLY

ASN

GLN

TYR

ASN

VAL

8

THR

TYR

VAL

LYS

GLU

ARG

GLY

ASP

TYR

9

VAL

LEU

ALA

VAL

LYS

TRP

GLY

GLU

HIS

10

ILE

PRO

GLY

SER

PRO

PHE

HIS

VAL

THR

VAL

11

PRO

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz