BMRB Entry 10160

Name: Solution structure of the 17th filamin domain from human Filamin-B
Published: 2009-02-20

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PDB ID: 2eea
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10160
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 17th filamin domain from human Filamin-B

Deposition date:

2007-12-28

Original release date:

2009-02-20

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 17th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
filamin domain, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060619-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
filamin domain: GSSGSSGPESPLQFYVNYPN SGSVSAYGPGLVYGVANKTA TFTIVTEDAGEGGLDLAIEG PSKAEISCIDNKDGTCTVTY LPTLPGDYSILVKYNDKHIP GSPFTAKITDDSRRC

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	470
15N chemical shifts	105
1H chemical shifts	721

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-B	1

Entities:

Entity 1, Filamin-B 115 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

GLU

SER

2

PRO

LEU

GLN

PHE

TYR

VAL

ASN

TYR

PRO

ASN

3

SER

GLY

SER

VAL

SER

ALA

TYR

GLY

PRO

GLY

4

LEU

VAL

TYR

GLY

VAL

ALA

ASN

LYS

THR

ALA

5

THR

PHE

THR

ILE

VAL

THR

GLU

ASP

ALA

GLY

6

GLU

GLY

LEU

ASP

LEU

ALA

ILE

GLU

GLY

7

PRO

SER

LYS

ALA

GLU

ILE

SER

CYS

ILE

ASP

8

ASN

LYS

ASP

GLY

THR

CYS

THR

VAL

THR

TYR

9

LEU

PRO

THR

LEU

PRO

GLY

ASP

TYR

SER

ILE

10

LEU

VAL

LYS

TYR

ASN

ASP

LYS

HIS

ILE

PRO

11

GLY

SER

PRO

PHE

THR

ALA

LYS

ILE

THR

ASP

12

ASP

SER

ARG

CYS

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz