BMRB Entry 10113

Name: Solution Structure of the Pleckstrin Homology Domain of Mouse APS
Published: 2008-08-15

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PDB ID: 1v5m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10113
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Pleckstrin Homology Domain of Mouse APS

Deposition date:

2007-02-15

Original release date:

2008-08-15

Authors:

Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Pleckstrin Homology Domain of Mouse APS" .

Assembly members:

Assembly members:
Pleckstrin Homology Domain, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030212-26

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pleckstrin Homology Domain: GSSGSSGNLAAKVELVDIQR EGALRFMVADDAASGPGGTA QWQKCRLLLRRAVAGERFRL EFFVPPKASRPKVSIPLSAI IEVRTTMPLEMPEKDNTFVL KVENGAEYILETIDSLQKHS WVADIQGCVDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	556
15N chemical shifts	125
1H chemical shifts	909

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH2 and PH domain-containing adapter protein APS	1

Entities:

Entity 1, SH2 and PH domain-containing adapter protein APS 136 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

LEU

ALA

2

ALA

LYS

VAL

GLU

LEU

VAL

ASP

ILE

GLN

ARG

3

GLU

GLY

ALA

LEU

ARG

PHE

MET

VAL

ALA

ASP

4

ASP

ALA

SER

GLY

PRO

GLY

THR

ALA

5

GLN

TRP

GLN

LYS

CYS

ARG

LEU

ARG

6

ARG

ALA

VAL

ALA

GLY

GLU

ARG

PHE

ARG

LEU

7

GLU

PHE

VAL

PRO

LYS

ALA

SER

ARG

8

PRO

LYS

VAL

SER

ILE

PRO

LEU

SER

ALA

ILE

9

ILE

GLU

VAL

ARG

THR

MET

PRO

LEU

GLU

10

MET

PRO

GLU

LYS

ASP

ASN

THR

PHE

VAL

LEU

11

LYS

VAL

GLU

ASN

GLY

ALA

GLU

TYR

ILE

LEU

12

GLU

THR

ILE

ASP

SER

LEU

GLN

LYS

HIS

SER

13

TRP

VAL

ALA

ASP

ILE

GLN

GLY

CYS

VAL

ASP

14

SER

GLY

PRO

SER

GLY

Samples:

sample_1: Pleckstrin Homology domain, [U-13C; U-15N], 1.23 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1V5M
DBJ	BAE24331 BAE24336
GB	AAC64408 AAF37891 AAH57334 EDL19313 EDL19314
REF	NP_001289867 NP_001289868 NP_061295 NP_446121 XP_005080443
SP	Q9JID9 Q9Z200
AlphaFold	Q9JID9 Q9Z200