BMRB Entry 10104

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PDB ID: 1ujx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10104
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The forkhead associated (FHA) domain like structure from mouse polynucleotide kinase 3'-phosphatase

Deposition date:

2007-02-13

Original release date:

2008-08-15

Authors:

Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "The forkhead associated (FHA) domain like structure from mouse polynucleotide kinase 3'-phosphatase" .

Assembly members:

Assembly members:
polynucleotide kinase 3'-phosphatase, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030107-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
polynucleotide kinase 3'-phosphatase: GSSGSSGMSQLGSRGRLWLQ SPTGGPPPIFLPSDGQALVL GRGPLTQVTDRKCSRNQVEL IADPESRTVAVKQLGVNPST VGVQELKPGLSGSLSLGDVL YLVNGLYPLTLRWSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	502
15N chemical shifts	122
1H chemical shifts	814

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polynucleotide kinase 3'-phosphatase	1

Entities:

Entity 1, polynucleotide kinase 3'-phosphatase 119 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

SER

GLN

2

LEU

GLY

SER

ARG

GLY

ARG

LEU

TRP

LEU

GLN

3

SER

PRO

THR

GLY

PRO

ILE

PHE

4

LEU

PRO

SER

ASP

GLY

GLN

ALA

LEU

VAL

LEU

5

GLY

ARG

GLY

PRO

LEU

THR

GLN

VAL

THR

ASP

6

ARG

LYS

CYS

SER

ARG

ASN

GLN

VAL

GLU

LEU

7

ILE

ALA

ASP

PRO

GLU

SER

ARG

THR

VAL

ALA

8

VAL

LYS

GLN

LEU

GLY

VAL

ASN

PRO

SER

THR

9

VAL

GLY

VAL

GLN

GLU

LEU

LYS

PRO

GLY

LEU

10

SER

GLY

SER

LEU

SER

LEU

GLY

ASP

VAL

LEU

11

TYR

LEU

VAL

ASN

GLY

LEU

TYR

PRO

LEU

THR

12

LEU

ARG

TRP

SER

GLY

PRO

SER

GLY

Samples:

sample_1: FHA domain, [U-13C; U-15N], 1.16 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker Biospin - collection

NMRPipe v20020425, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.0.4, Johnson, One Moon Scientific - data analysis

Kujira v0.851, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

Bruker Avance 800 MHz

PDB	1UJX 1YJ5 1YJM
DBJ	BAE43066
GB	AAF36487 AAH57659 EDL22761 EDL22763
REF	NP_001277693 NP_001277695 NP_067524 XP_006541101 XP_006541102
SP	Q9JLV6
AlphaFold	Q9JLV6