BMRB Entry 10075

Name: The fourth FN3 domain of human sidekick-2
Published: 2008-08-14

Click here to enlarge.

PDB ID: 1wfn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10075
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

The fourth FN3 domain of human sidekick-2

Deposition date:

2006-12-25

Original release date:

2008-08-14

Authors:

Nagashima, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "The fourth FN3 domain of human sidekick-2" .

Assembly members:

Assembly members:
FN3 domain, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040223-46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FN3 domain: GSSGSSGPQLVRTHEDVPGP VGHLSFSEILDTSLKVSWQE PGEKNGILTGYRISWEEYNR TNTRVTHYLPNVTLEYRVTG LTALTTYTIEVAAMTSKGQG QVSASTISSGVPPSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	479
15N chemical shifts	113
1H chemical shifts	757

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sidekick 2	1

Entities:

Entity 1, sidekick 2 119 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

GLN

LEU

2

VAL

ARG

THR

HIS

GLU

ASP

VAL

PRO

GLY

PRO

3

VAL

GLY

HIS

LEU

SER

PHE

SER

GLU

ILE

LEU

4

ASP

THR

SER

LEU

LYS

VAL

SER

TRP

GLN

GLU

5

PRO

GLY

GLU

LYS

ASN

GLY

ILE

LEU

THR

GLY

6

TYR

ARG

ILE

SER

TRP

GLU

TYR

ASN

ARG

7

THR

ASN

THR

ARG

VAL

THR

HIS

TYR

LEU

PRO

8

ASN

VAL

THR

LEU

GLU

TYR

ARG

VAL

THR

GLY

9

LEU

THR

ALA

LEU

THR

TYR

THR

ILE

GLU

10

VAL

ALA

MET

THR

SER

LYS

GLY

GLN

GLY

11

GLN

VAL

SER

ALA

SER

THR

ILE

SER

GLY

12

VAL

PRO

SER

GLY

PRO

SER

GLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.21 mM; d-TRIS 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 900 MHz
Varian INOVA 800 MHz