BMRB Entry 10074

Title:
The first C2 domain of human synaptotagmin XIII
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "The first C2 domain of human synaptotagmin XIII"  .

Assembly members:

Assembly members:
C2 domain, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040113-87

Data sets:
Data typeCount
13C chemical shifts549
15N chemical shifts126
1H chemical shifts861

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1synaptotagmin XIII1

Entities:

Entity 1, synaptotagmin XIII 138 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERTRPASN
2   GLNALAPROLYSLEUHISTYRCYSLEUASP
3   TYRASPCYSGLNLYSALAGLULEUPHEVAL
4   THRARGLEUGLUALAVALTHRSERASNHIS
5   ASPGLYGLYCYSASPCYSTYRVALGLNGLY
6   SERVALALAASNARGTHRGLYSERVALGLU
7   ALAGLNTHRALALEULYSLYSARGGLNLEU
8   HISTHRTHRTRPGLUGLUGLYLEUVALLEU
9   PROLEUALAGLUGLUGLULEUPROTHRALA
10   THRLEUTHRLEUTHRLEUARGTHRCYSASP
11   ARGPHESERARGHISSERVALALAGLYGLU
12   LEUARGLEUGLYLEUASPGLYTHRSERVAL
13   PROLEUGLYALAALAGLNTRPGLYGLULEU
14   LYSTHRSERGLYPROSERSERGLY

Samples:

sample_1: C2 domain, [U-13C; U-15N], 1.07 mM; d-TRIS 20 mM; NaCl 100 mM; d-DTT 5 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - processing, refinement

NMR spectrometers:

  • Varian INOVA 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks