BMRB Entry 10061

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10061
MolProbity Validation Chart

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Title:
Solution structure of PDZ domain of mouse Cypher protein
Deposition date:
2006-11-29
Original release date:
2008-08-13
Authors:
Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of PDZ domain of mouse Cypher protein"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P031104-22

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGMSYSVTLTGPGPW GFRLQGGKDFNMPLTISRIT PGSKAAQSQLSQGDLVVAID GVNTDTMTHLEAQNKIKSAS YNLSLTLQKS

Data sets:
Data typeCount
13C chemical shifts371
15N chemical shifts94
1H chemical shifts599

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cypher protein1

Entities:

Entity 1, Cypher protein 90 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETSERTYR
2   SERVALTHRLEUTHRGLYPROGLYPROTRP
3   GLYPHEARGLEUGLNGLYGLYLYSASPPHE
4   ASNMETPROLEUTHRILESERARGILETHR
5   PROGLYSERLYSALAALAGLNSERGLNLEU
6   SERGLNGLYASPLEUVALVALALAILEASP
7   GLYVALASNTHRASPTHRMETTHRHISLEU
8   GLUALAGLNASNLYSILELYSSERALASER
9   TYRASNLEUSERLEUTHRLEUGLNLYSSER

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.1 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8996, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA31588 BAB23128 BAD32258 BAE23262 BAE25016
EMBL CAB46727 CAB46728 CAB46729 CAB46747
GB AAD42950 AAD42951 AAF33847 AAF33848 AAH10929
REF NP_001030493 NP_001034160 NP_001034161 NP_001034162 NP_001034163
SP O75112 Q9JKS4
TPG DAA14160
AlphaFold O75112 Q9JKS4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks