BMRB Entry 10050

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10050
MolProbity Validation Chart

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Title:
Solution structure of N-terminal ubiquitin-like domain of human NEDD8 ultimate buster-1
Deposition date:
2006-11-09
Original release date:
2008-08-13
Authors:
Yoneyama, M.; Koshiba, S.; Tochio, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Koshiba, S.; Tochio, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of N-terminal ubiquitin-like domain of human NEDD8 ultimate buster-1"  .

Assembly members:

Assembly members:
Ubiquitin-like domain, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040301-74

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin-like domain: GSSGSSGDNYRTTGIATIEV FLPPRLKKDRKNLLETRLHI TGRELRSKIAETFGLQENYI KIVINKKQLQLGKTLEEQGV AHNVKAMVLELKQSSGPSSG

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts86
1H chemical shifts661

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NEDD8 ultimate buster-11

Entities:

Entity 1, NEDD8 ultimate buster-1 100 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPASNTYR
2   ARGTHRTHRGLYILEALATHRILEGLUVAL
3   PHELEUPROPROARGLEULYSLYSASPARG
4   LYSASNLEULEUGLUTHRARGLEUHISILE
5   THRGLYARGGLULEUARGSERLYSILEALA
6   GLUTHRPHEGLYLEUGLNGLUASNTYRILE
7   LYSILEVALILEASNLYSLYSGLNLEUGLN
8   LEUGLYLYSTHRLEUGLUGLUGLNGLYVAL
9   ALAHISASNVALLYSALAMETVALLEUGLU
10   LEULYSGLNSERSERGLYPROSERSERGLY

Samples:

sample_1: Ubiquitin-like domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Jhonson, B.A. - data analysis

Kujira v0.896, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

xwinnmr v3.5, Bruker - collection

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

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