BMRB Entry 10040

Title:
Solution Structure of the RA Domain of Human Grb7 Protein
Deposition date:
2006-11-09
Original release date:
2008-08-13
Authors:
Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RA Domain of Human Grb7 Protein"  .

Assembly members:

Assembly members:
RA domain, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040223-63

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts95
1H chemical shifts609

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Growth factor receptor-bound protein 71

Entities:

Entity 1, Growth factor receptor-bound protein 7 100 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGPROHIS
2   VALVALLYSVALTYRSERGLUASPGLYALA
3   CYSARGSERVALGLUVALALAALAGLYALA
4   THRALAARGHISVALCYSGLUMETLEUVAL
5   GLNARGALAHISALALEUSERASPGLUTHR
6   TRPGLYLEUVALGLUCYSHISPROHISLEU
7   ALALEUGLUARGGLYLEUGLUASPHISGLU
8   SERVALVALGLUVALGLNALAALATRPPRO
9   VALGLYGLYASPSERARGPHEVALPHEARG
10   LYSASNPHEALASERGLYPROSERSERGLY

Samples:

sample_1: RA domain, [U-13C, U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.897, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA07827 BAA13412 BAA29059 BAA29060 BAD96569
GB AAG25938 AAH06535 AAP35332 AAP36681 AAX29563
REF NP_001025173 NP_001229371 NP_001229372 NP_005301 XP_001171807
SP Q14451
AlphaFold Q14451

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks