BMRB Entry 10038

Name: Solution structure of zf-C2H2 domains from human Zinc finger protein 295
Published: 2008-08-12

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PDB ID: 1wjp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10038
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of zf-C2H2 domains from human Zinc finger protein 295

Deposition date:

2006-11-02

Original release date:

2008-08-12

Authors:

Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of zf-C2H2 domains from human Zinc finger protein 295" .

Assembly members:

Assembly members:
zf-C2H2 domain, polymer, 107 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040126-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zf-C2H2 domain: GSSGSSGASPVENKEVYQCR LCNAKLSSLLEQGSHERLCR NAAVCPYCSLRFFSPELKQE HESKCEYKKLTCLECMRTFK SSFSIWRHQVEVHNQNNMAP TSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	447
15N chemical shifts	106
1H chemical shifts	689

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger protein 295	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2
4	ZINC (II) ION, 3	2

Entities:

Entity 1, Zinc finger protein 295 107 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

SER

PRO

2

VAL

GLU

ASN

LYS

GLU

VAL

TYR

GLN

CYS

ARG

3

LEU

CYS

ASN

ALA

LYS

LEU

SER

LEU

4

GLU

GLN

GLY

SER

HIS

GLU

ARG

LEU

CYS

ARG

5

ASN

ALA

VAL

CYS

PRO

TYR

CYS

SER

LEU

6

ARG

PHE

SER

PRO

GLU

LEU

LYS

GLN

GLU

7

HIS

GLU

SER

LYS

CYS

GLU

TYR

LYS

LEU

8

THR

CYS

LEU

GLU

CYS

MET

ARG

THR

PHE

LYS

9

SER

PHE

SER

ILE

TRP

ARG

HIS

GLN

VAL

10

GLU

VAL

HIS

ASN

GLN

ASN

MET

ALA

PRO

11

THR

SER

GLY

PRO

SER

GLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zf-C2H2 domain, [U-13C; U-15N], 1.03 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; ZnCl2 0.01 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_13C-separated_NOESY	sample_1	not available	condition_1
3D_15N-separated_NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WJP
DBJ	BAA86541 BAA95529 BAD74063 BAD74064 BAE02111
GB	AAH63290 AIC56336 EAX09579 EAX09580 EAX09581
REF	NP_001091872 NP_001091873 NP_065778 XP_001106387 XP_001106513
SP	Q9ULJ3
AlphaFold	Q9ULJ3