BMRB Entry 10015

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR10015

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Assignments of a HIV-1 integrase Zn finger domain mutant
Deposition date:
2005-12-04
Original release date:
2007-02-07
Authors:
Nomura, Yusuke; Masuda, Takao; Kawai, Gota
Citation:

Citation: Nomura, Yusuke; Masuda, Takao; Kawai, Gota. "Structural Analysis of a Mutant of the HIV-1 Integrase Zinc Finger Domain That Forms a Single Conformation"  J. Biochem. (Tokyo) 139, 753-759 (2006).
PubMed: 16672276

Assembly members:

Assembly members:
Y15A, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human immunodeficiency virus   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus Human immunodeficiency virus 1

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Y15A: FLDGIDKAQEEHEKAHSNWR AMASDFNLPPVVAKEIVASC DKCQLKGEAMHGQVD

Data sets:
Data typeCount
13C chemical shifts130
15N chemical shifts48
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1integrase Zn finger domain mutant1
2ZINC (II) ION2

Entities:

Entity 1, integrase Zn finger domain mutant 55 residues - Formula weight is not available

1   PHELEUASPGLYILEASPLYSALAGLNGLU
2   GLUHISGLULYSALAHISSERASNTRPARG
3   ALAMETALASERASPPHEASNLEUPROPRO
4   VALVALALALYSGLUILEVALALASERCYS
5   ASPLYSCYSGLNLEULYSGLYGLUALAMET
6   HISGLYGLNVALASP

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

y15a: Y15A, [U-13C; U-15N], 1.8 ± 0.1 mM

condition_1: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCy15anot availablecondition_1
1H-13C HSQCy15anot availablecondition_1
HBHACONHy15anot availablecondition_1
HNCAy15anot availablecondition_1
HN(CO)CAy15anot availablecondition_1
CBCANHy15anot availablecondition_1
CBCA(CO)NHy15anot availablecondition_1
HNCOy15anot availablecondition_1
HNCACOy15anot availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAA00993 BAB85751 BAF34642 BAG31016 BAG31025
EMBL CAA06947 CAA77622 CAB58981 CBM41433 CDX10560
GB AAA44653 AAA44988 AAA83392 AAB04037 AAB47928
SP P04587 P12497 P20892 P35963 Q9QBZ1
AlphaFold P04587 P12497 P20892 P35963 Q9QBZ1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks