BMRB Entry 7028

Title:
Chemical shifts of the selected resonances in calmodulin alone and in complex with various sk-MLCK target peptides
Deposition date:
2005-08-31
Original release date:
2013-02-15
Authors:
Findlay, Wendy; Gradwell, Michael; Bayley, Peter
Citation:

Citation: Findlay, Wendy; Gradwell, Michael; Bayley, Peter. "Role of the N-terminal region of the skeletal muscle myosin light chain kinase target sequence in its interaction with calmodulin"  Protein Sci. 4, 2375-2382 (1995).
PubMed: 8563635

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available
WFF peptide, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2WFF2

Entities:

Entity 1, CaM 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHETYRGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYPHEILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, WFF 18 residues - Formula weight is not available

1   LYSARGARGTRPLYSLYSASNPHEILEALA
2   VALSERALAALAASNARGPHELYS

Samples:

sample_1: calmodulin 1 mM; Tris 10 mM; KCl 100 mM; CaCl2 10 mM; WFF peptide 1 mM

conditions_1: pH*: 7.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
unknownsample_1isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6541 6778 6798 6802 6825 6830 6831 7018 7029 7030 7031 7416 7417 7418 7423 7424 7425 18556
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAD30083 BAD30084 BAD30085 BAD30086 BAI47180
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA46981 CAC81354
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA31400 AAC71016 AAC83671 AAH07753 AAH19408
PIR JC1305 MCON
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_001074513 NP_001075705 NP_001077188 NP_149109 XP_001499833
SP O02367 O16305 O96081 P02594 P05932 A4IFM7 P07313 P20689 Q8VCR8 Q9H1R3
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA23271
PRF 1507147A
AlphaFold P02594 O02367 O16305 O96081 P05932 A4IFM7 Q9H1R3 Q8VCR8 P20689 P07313