BMRB Entry 6917

Title:
Backbone assignments of Pyrococcus furiosus Pop5 (PF1378), an archaeal RNase P protein.
Deposition date:
2005-12-05
Original release date:
2006-01-23
Authors:
Wilson, Ross; Foster, Mark
Citation:

Citation: Wilson, Ross; Bohlen, C.; Foster, Mark; Bell, C. "Structure of Pfu Pop5, an archaeal RNase P protein"  Proc. Natl. Acad. Sci. U.S.A. 103, 873-878 (2006).
PubMed: 16418270

Assembly members:

Assembly members:
Pfu pop5, polymer, 120 residues, 13708 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts306
15N chemical shifts102
1H chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pop5 monomer1

Entities:

Entity 1, pop5 monomer 120 residues - 13708 Da.

1   METSERGLUARGPROLYSTHRLEUPROPRO
2   THRLEUARGASPLYSLYSARGTYRILEALA
3   PHELYSVALILESERGLUASNGLNPHEASN
4   LYSASPGLUILELYSGLUALAILETRPASN
5   ALACYSLEUARGTHRLEUGLYGLULEUGLY
6   THRALALYSALALYSPROTRPLEUILELYS
7   PHEASPGLUTHRTHRGLNTHRGLYILEILE
8   ARGSERASPARGASNHISVALTYRASPVAL
9   ILEPHESERLEUTHRLEUVALSERASPILE
10   ASNGLYASNLYSALAILEILELYSVALLEU
11   GLYVALSERGLYTHRILELYSARGLEULYS
12   ARGLYSPHELEUSERGLNPHEGLYTRPARG

Samples:

sample_1: Pfu pop5, [U-95% 13C; U-95% 15N], 0.3 mM; Sodium Acetate 10 mM; Potassium Chloride 0.5 mM; Sodium Azide 0.05 mM

conditions_1: pH: 5.0; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H1513C HNCOsample_1not availableconditions_1
1H1513C HNCAsample_1not availableconditions_1
1H1513C HNCACBsample_1not availableconditions_1
1H1513C CBCACONHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance DRX 600 MHz

Related Database Links:

BMRB 17190
PDB
GB AAL81502 AFN04159
REF NP_579107 WP_011012525 YP_006492451
SP Q8U151
AlphaFold Q8U151

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks