BMRB Entry 6851

Title:
Solution Structure of the human homodimeric DNA repair protein XPF.
Deposition date:
2005-10-05
Original release date:
2007-11-21
Authors:
Das, Devashish; Tripsianes, Konstantinos; Kaptein, Rob; Folkers, Gert; Bolenes, Rolf
Citation:

Citation: Das, Devashish; Tripsianes, Konstantinos; Jaspers, N.; Hoeijmakers, J.; Kaptein, R.; Boelens, R.; Folkers, G.. "The HhH domain of the human DNA repair protein XPF forms stable homodimers."  Proteins 70, 1551-1563 (2008).
PubMed: 17912758

Assembly members:

Assembly members:
XPF, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts87
1H chemical shifts436

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1XPF dimer protein unit A1
2XPF dimer protein unit B1

Entities:

Entity 1, XPF dimer protein unit A 84 residues - Formula weight is not available

1   METASPSERGLUTHRLEUPROGLUSERGLU
2   LYSTYRASNPROGLYPROGLNASPPHELEU
3   LEULYSMETPROGLYVALASNALALYSASN
4   CYSARGSERLEUMETHISHISVALLYSASN
5   ILEALAGLULEUALAALALEUSERGLNASP
6   GLULEUTHRSERILELEUGLYASNALAALA
7   ASNALALYSGLNLEUTYRASPPHEILEHIS
8   THRSERPHEALAGLUVALVALSERLYSGLY
9   LYSGLYLYSLYS

Samples:

13C-15N_label: XPF, [U-98 % 13C; U-98 % 15N], 1 ± 0.1 mM; Nacl 400 mM; phosphate 10 mM

400mM_Nacl_10mM_phosphate_pH-5: pH*: 5; temperature: 293.67 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQC13C-15N_labelnot available400mM_Nacl_10mM_phosphate_pH-5
triple resonance experiment13C-15N_labelnot available400mM_Nacl_10mM_phosphate_pH-5
heteronuclear NOE13C-15N_labelnot available400mM_Nacl_10mM_phosphate_pH-5
homonuclear NOE13C-15N_labelnot available400mM_Nacl_10mM_phosphate_pH-5

Software:

xwinnmr -

SPARKY -

NMRPipe -

CYANA -

CNS -

NMR spectrometers:

  • Bruker DRX, Avance 600 MHz
  • Bruker DRX, Avance 700 MHz

Related Database Links:

BMRB 6551
PDB
DBJ BAF82415 BAG63350
GB AAB07689 AAB50174 AAI60102 AAL91593 EAW85117
REF NP_005227 XP_003832737 XP_004057285 XP_510831
SP Q92889
AlphaFold Q92889

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks