BMRB Entry 6634

Title:
Chemical shift assignments for the K45Q-mutated ferricytochrome c3 from Desulfovibrio vulgaris Hildenborough
Deposition date:
2005-05-13
Original release date:
2007-01-26
Authors:
Messias, Ana; Aguiar, Antonio; Brennan, Lorraine; Salgueiro, Carlos; Saraiva, Ligia; Xavier, Antonio; Turner, David
Citation:

Citation: Messias, Ana; Aguiar, Antonio; Brennan, Lorraine; Salgueiro, Carlos; Saraiva, Ligia; Xavier, Antonio; Turner, David. "Solution structures of tetrahaem ferricytochrome c(3) from Desulfovibrio vulgaris (Hildenborough) and its K45Q mutant: The molecular basis of cooperativity"  Biochim. Biophys. Acta. 1757, 143-153 (2006).
PubMed: 16527248

Assembly members:

Assembly members:
K45Q cytochrome c3, polymer, 107 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Desulfovibrio vulgaris Hildenborough   Taxonomy ID: 881   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Desulfovibrio vulgaris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Desulfovibrio desulfuricans   Vector: pJRD215

Data typeCount
1H chemical shifts659

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K45Q cytochrome c31
2heme, I2
3heme, II2
4heme, III2
5heme, IV2

Entities:

Entity 1, K45Q cytochrome c3 107 residues - Formula weight is not available

1   ALAPROLYSALAPROALAASPGLYLEULYS
2   METGLUALATHRLYSGLNPROVALVALPHE
3   ASNHISSERTHRHISLYSSERVALLYSCYS
4   GLYASPCYSHISHISPROVALASNGLYLYS
5   GLUASPTYRARGGLNCYSGLYTHRALAGLY
6   CYSHISASPSERMETASPLYSLYSASPLYS
7   SERALALYSGLYTYRTYRHISVALMETHIS
8   ASPLYSASNTHRLYSPHELYSSERCYSVAL
9   GLYCYSHISVALGLUVALALAGLYALAASP
10   ALAALALYSLYSLYSASPLEUTHRGLYCYS
11   LYSLYSSERLYSCYSHISGLU

Entity 2, heme, I - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: K45Q cytochrome c3 0.85 mM; ampicillin 127 uM; kanamicin 78 uM; chloramphenicol 141 uM; H2O 92%; D2O 8%

conditions_1: pH*: 7.1; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-NOESYsample_1not availableconditions_1
2D-1H-TOCSYsample_1not availableconditions_1
2D-1H-COSYsample_1not availableconditions_1

Software:

XEASY v1.2 -

NMR spectrometers:

  • BRUKER DRX 500 MHz

Related Database Links:

BMRB 4291 5239 5625
PDB
EMBL CAA27847
GB AAS97641 ABM27239 ADP88070
REF WP_010940429 YP_005703708 YP_012381 YP_965666
SP P00131
AlphaFold P00131