BMRB Entry 4931

Title:
Solution structure of dynein light chain 8 (DLC8) and bim peptide complex
Deposition date:
2000-12-21
Original release date:
2001-08-22
Authors:
Fan, Jing-Song.; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie
Citation:

Citation: Fan, Jing-song.; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8 kDa Dynein Light Chain "  J. Mol. Biol. 306, 97-108 (2001).
PubMed: 11178896

Assembly members:

Assembly members:
DYNEIN light chain 8, polymer, 89 residues, 10300 Da.
Bcl-2, polymer, 9 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET14B

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts608
13C chemical shifts259
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dynein unit 11
2dynein unit 21
3Bim unit 12
4Bim unit 22

Entities:

Entity 1, dynein unit 1 89 residues - 10300 Da.

1   METCYSASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   SERVALGLUCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   HISILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   LYSHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Entity 2, Bim unit 1 9 residues - Formula weight is not available

1   METSERCYSASPLYSSERTHRGLNTHR

Samples:

sample_1: DYNEIN light chain 8, [U-15N; U-13C], 1.5 mM; Bcl-2 1.5 mM

sample_cond_1: pH: 7.0; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

VNMR v6.1b - data collection

NMRPipe v1.7 - data processing

X-PLOR v3.8 - structure solution

CNS v1.0 - refinement

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

SWISS-PROT P63169 P63168 P63167 P61285 P61273
REF NP_003737 NP_001075487 NP_001032584 NP_001032583 NP_001003901
GenBank AAD01643 AAC32531 AAC32530 AAB38257 AAB04149
EMBL CAG46925 CAG28600
DBJ BAB28973 BAB28970 BAB27117 BAB27063 BAB22160
PDB
BMRB 4912 4911 4305

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