BMRB Entry 4769

Title:
Backbone 1H, 13C and 15N assignments for yeast ubiquitin at pH 7.5
Deposition date:
2000-06-22
Original release date:
2001-05-03
Authors:
Hamilton, Katherine; Ellison, Michael; Shaw, Gary
Citation:

Citation: Hamilton, Katherine; Ellison, Michael; Shaw, Gary. "Identification of the Ubiquitin Interfacial Residues in a Ubiquitin-E2 Covalent Complex"  J. Biomol. NMR 18, 319-327 (2000).
PubMed: 11200526

Assembly members:

Assembly members:
yeast ubiquitin, polymer, 76 residues, 8500 Da.

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts133
15N chemical shifts76
1H chemical shifts321

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yeast ubiquitin1

Entities:

Entity 1, yeast ubiquitin 76 residues - 8500 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUSERSER
3   ASPTHRILEASPASNVALLYSSERLYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYARGGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: yeast ubiquitin, [U-95% 13C; U-90% 15N], 0.8 mM; HEPES 40 mM; NaCl 50 mM; EDTA 1 mM

condition1: pH: 7.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 15592 17024 4983
PDB
DBJ BAA76889 BAA88168 BAB79488 BAD97865 BAE57827
EMBL CAA07773 CAA11267 CAA21278 CAA25705 CAA25706
GB AAA03351 AAA36787 AAA42855 AAA56880 AAA72565
PRF 1101405A
REF NP_012118 NP_013020 NP_013061 NP_013268 NP_593923
SP P05759 P0C016 P0C224 P0C8R3 P0CG63
TPD FAA00316 FAA00317 FAA00318 FAA00319
TPE CBF76581 CBF85986
TPG DAA08405 DAA09244 DAA09283 DAA09489
AlphaFold P0C016 P0CG63 P0C8R3 P05759 P0C224

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks