BMRB Entry 4553

Title:
Assignment of 1H, 13C and 15N Resonances of the I-domain of Human Leukocyte Function Associated Antigen-1
Deposition date:
1999-12-10
Original release date:
2000-12-12
Authors:
Kriwacki, Richard; Legge, Glen; Hommel, Ulrich; Ramage, Paul; Chung, John; Tennant, Linda; Wright, Peter; Dyson, H.
Citation:

Citation: Kriwacki, Richard; Legge, Glen; Hommel, Ulrich; Ramage, Paul; Chung, John; Tennant, Linda; Wright, Peter; Dyson, H.. "Assignment of 1H, 13C and 15N Resonances of the I-domain of Human Leukocyte Function Associated Antigen-1"  J. Biomol. NMR 16, 271-272 (2000).

Assembly members:

Assembly members:
Leukocyte Function Associated Antigen-1 Inserted-Domain, polymer, 189 residues, 21268 Da.
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET17b

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts690
15N chemical shifts191
1H chemical shifts1392

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LFA-11
2magnesium2

Entities:

Entity 1, LFA-1 189 residues - 21268 Da.

1   METALASERLYSGLYASNVALASPLEUVAL
2   PHELEUPHEASPGLYSERMETSERLEUGLN
3   PROASPGLUPHEGLNLYSILELEUASPPHE
4   METLYSASPVALMETLYSLYSLEUSERASN
5   THRSERTYRGLNPHEALAALAVALGLNPHE
6   SERTHRSERTYRLYSTHRGLUPHEASPPHE
7   SERASPTYRVALLYSTRPLYSASPPROASP
8   ALALEULEULYSHISVALLYSHISMETLEU
9   LEULEUTHRASNTHRPHEGLYALAILEASN
10   TYRVALALATHRGLUVALPHEARGGLUGLU
11   LEUGLYALAARGPROASPALATHRLYSVAL
12   LEUILEILEILETHRASPGLYGLUALATHR
13   ASPSERGLYASNILEASPALAALALYSASP
14   ILEILEARGTYRILEILEGLYILEGLYLYS
15   HISPHEGLNTHRLYSGLUSERGLNGLUTHR
16   LEUHISLYSPHEALASERLYSPROALASER
17   GLUPHEVALLYSILELEUASPTHRPHEGLU
18   LYSLEULYSASPLEUPHETHRGLULEUGLN
19   LYSLYSILETYRVALILEGLUGLYMET

Entity 2, magnesium - Mg - 24.305 Da.

1   MG

Samples:

sample_1: Leukocyte Function Associated Antigen-1 Inserted-Domain, [U-13C; U-15N], 1.2 mM; NaH2PO4 10 mM; MgSO4 10 mM; NaCl 150 mM; NaN3 0.05%; 2H2O 5%

sample_2: Leukocyte Function Associated Antigen-1 Inserted-Domain, [U-15N], 1.2 mM

condition_1: ionic strength: 0.28 M; pH*: 7.2; temperature: 295 K

condition_2: ionic strength: 0.018 M; pH*: 7.3 na; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
CC(CO)NH TOSCYnot availablenot availablenot available
HCCH COSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HCCH TOCSY (aromatic)not availablenot availablenot available
2D 13C HSQCnot availablenot availablenot available
3D HB(CBCG)CDHDnot availablenot availablenot available

Software:

FELIX v97 -

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz
  • Bruker AMX 500 MHz

Related Database Links:

PDB
DBJ BAG36913
EMBL CAA68747
GenBank AAC31672 AAQ14923 AAZ38713 EAW52244 EAW52245
REF NP_001029341 NP_002200
SWISS-PROT P20701

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks