BMRB Entry 4228

Title:
NMR structures (20) of the J-Domain (residues 1-77) of the Escherichia coli n-terminal fragment (residues 1-104) of the molecular chaperone DNAJ
Deposition date:
1998-08-20
Original release date:
2001-07-09
Authors:
Huang, K.; Flanagan, J.; Prestegard, J.
Citation:

Citation: Huang, K.; Flanagan, J.; Prestegard, J.. "The influence of c-terminal extension on the structure of the j-domain in e. coli dnaj""  Protein Sci. ., 5587-5596 (1998).

Assembly members:

Assembly members:
DNAJ, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: Proteobacteria   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: T7

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts92
1H chemical shifts600

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNAJ1

Entities:

Entity 1, DNAJ 103 residues - Formula weight is not available

1   ALALYSGLNASPTYRTYRGLUILELEUGLY
2   VALSERLYSTHRALAGLUGLUARGGLUILE
3   ARGLYSALATYRLYSARGLEUALAMETLYS
4   TYRHISPROASPARGASNGLNGLYASPLYS
5   GLUALAGLUALALYSPHELYSGLUILELYS
6   GLUALATYRGLUVALLEUTHRASPSERGLN
7   LYSARGALAALATYRASPGLNTYRGLYHIS
8   ALAALAPHEGLUGLNGLYGLYMETGLYGLY
9   GLYGLYPHEGLYGLYGLYALAASPPHESER
10   ASPILEPHEGLYASPVALPHEGLYASPILE
11   PHEGLYGLY

Samples:

sample_1: DNAJ mM; phosphate 50 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 600 MHz
  • GE Omega 500 MHz

Related Database Links:

BMRB 4227
PDB
DBJ BAB33438 BAB96590 BAG75538 BAI23378 BAI28898
EMBL CAP74585 CAQ30532 CAQ87599 CAQ96906 CAR01382
GB AAA00009 AAA23693 AAC73126 AAG54315 AAN41681
REF NP_308042 NP_414556 NP_705974 WP_000240600 WP_001118445
SP A7ZHA5 A7ZVV8 B1IRF9 B1LFU5 B1XBE0
AlphaFold A7ZHA5 A7ZVV8 B1IRF9 B1LFU5 B1XBE0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks