BMRB Entry 4209

Title:
Solution NMR structures of the major coat protein of filamentous bacteriophage M13 solubilized in Dodecyl Phosphocholine micelles, 25 lowest energy structures
Deposition date:
1998-04-17
Original release date:
2000-02-03
Authors:
Papavoine, C.; Christiaans, B.; Folmer, R.; Konings, R.; Hilbers, C.
Citation:

Citation: Papavoine, C.; Christiaans, B.; Folmer, R.; Konings, R.; Hilbers, C.. "Solution structure of the M13 major coat protein in detergent micelles: A basis for a model of phage assembly involving specific residues"  J. Mol. Biol. 282, 401-419 (1998).

Assembly members:

Assembly members:
M13 major coat protein, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: bacteriophage M13   Taxonomy ID: 10870   Superkingdom: viruses   Kingdom: not available   Genus/species: Inoviridae Coliphage M13

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M13 major coat protein: AEGDDPAKAAFNSLQASATE YIGYAWAMVVVIVGATIGIK LFKKFTSKAS

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts50
1H chemical shifts317
coupling constants29

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gVIIIp1

Entities:

Entity 1, gVIIIp 50 residues - Formula weight is not available

1   ALAGLUGLYASPASPPROALALYSALAALA
2   PHEASNSERLEUGLNALASERALATHRGLU
3   TYRILEGLYTYRALATRPALAMETVALVAL
4   VALILEVALGLYALATHRILEGLYILELYS
5   LEUPHELYSLYSPHETHRSERLYSALASER

Samples:

sample_1: M13 major coat protein, [U-100% 13C; U-100% 15N], 1.5 – 2 mM; dodecyl phosphocholine

sample_cond_1: pH: 4.9; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
NOESY-HMQC (15N and 13C)not availablenot availablenot available
TOCSY-HMQC (15N)not availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
ROESY-GHSQCnot availablenot availablenot available
HMQC-NOESY-GHSQCnot availablenot availablenot available
HMQC-Jnot availablenot availablenot available
HNHAnot availablenot availablenot available
HACACB-COSYnot availablenot availablenot available
HNHBnot availablenot availablenot available
HSQC(3J CGN)not availablenot availablenot available
HSQC(3JCGC')not availablenot availablenot available

Software:

MNMR - FT of the recorded spectra

XEASY v3.9.1 - Analysis of the spectra to obtain assignments and structural information

NMR spectrometers:

  • Bruker AM 400 MHz
  • Bruker AM 500 MHz
  • Varian Unity+ 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 17728 19734 19747 2590 2591 2592 4197
PDB
EMBL CAA23861 CAA23871
GB AAA32207 AAA32214 AAA32220 AAA32308 AAB24445
PRF 0812197K
REF NP_510890 YP_009111292 YP_009111302
SP P69539 P69540 P69541
AlphaFold P69539 P69540 P69541

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks