BMRB Entry 4069

Title:
Secondary Structure Determination of 15N-Labelled Human Long-[Arg-3]-Insulin-Like Growth Factor-I by Multidimensional NMR Spectroscopy
Deposition date:
1997-10-28
Original release date:
2002-08-12
Authors:
Laajoki, Leanne; Le Breton, Eugene; Shooter, Gary; Wallace, John; Francis, Geoff; Carver, John; Keniry, Max
Citation:

Citation: Laajoki, Leanne; Le Breton, Eugene; Shooter, Gary; Wallace, John; Francis, Geoff; Carver, John; Keniry, Max. "Secondary Structure Determination of 15N-Labelled Human Long-[Arg-3]-Insulin-Like Growth Factor-I by Multidimensional NMR Spectroscopy"  FEBS Lett. ., .-. (1997).

Assembly members:

Assembly members:
Long-[Arg-3]-Insulin-Like Growth Factor-I, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p[Met1]-pGH(11)-Val-Asn-IGF-I

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Long-[Arg-3]-Insulin-Like Growth Factor-I: MFPAMPLSSLFVNGPRTLCG AELVDALQFVCGDRGFYFNK PTGYGSSSRRAPQTGIVDEC CFRSCDLRRLEMYCAPLKPA KSA

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts549

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Long-[Arg-3]-IGF-I1

Entities:

Entity 1, Long-[Arg-3]-IGF-I 83 residues - Formula weight is not available

1   METPHEPROALAMETPROLEUSERSERLEU
2   PHEVALASNGLYPROARGTHRLEUCYSGLY
3   ALAGLULEUVALASPALALEUGLNPHEVAL
4   CYSGLYASPARGGLYPHETYRPHEASNLYS
5   PROTHRGLYTYRGLYSERSERSERARGARG
6   ALAPROGLNTHRGLYILEVALASPGLUCYS
7   CYSPHEARGSERCYSASPLEUARGARGLEU
8   GLUMETTYRCYSALAPROLEULYSPROALA
9   LYSSERALA

Samples:

sample_one: Long-[Arg-3]-Insulin-Like Growth Factor-I, [U-15N], 1.0 mM; H2O 90%; D2O 10%

sample_two: Long-[Arg-3]-Insulin-Like Growth Factor-I 1.3 mM; D2O 100%

sample_conditions_IGF-I: pH: 4.1; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian INOVA-500 500 MHz
  • Varian INOVA-600 600 MHz

Related Database Links:

BMRB 15654 2498 4204 4278 4494
PDB
DBJ BAA01200 BAA07897
EMBL CAA27153 CAA28759 CAA35098
GB AAA31544 AAA53535 AAA72202 AAA72204 AAA72455
PIR S22878
PRF 0912651A 1707201A 2001274B
REF NP_001300784 XP_004418088 XP_004418089 XP_004418090 XP_004418091
SP P33712 Q28933
AlphaFold P33712 Q28933

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks