Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25586
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Citation: Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Sklan, Ella; Willbold, Dieter; Koenig, Bernd. "Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes." Viruses 7, 4119-4130 (2015).
PubMed: 26197333
Assembly members:
Molecule_1, polymer, 48 residues, 5226.9633 Da.
Natural source: Common Name: DENV Type 2 Taxonomy ID: 56636 Superkingdom: Archaea Kingdom: not available Genus/species: Aeropyrum pernix
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-2
Entity Sequences (FASTA):
Molecule_1: SLTLNLITEMGRLPTFMTQK
ARDALDNLAVLHTAEAGGRA
YNHALSEL
Data type | Count |
13C chemical shifts | 92 |
15N chemical shifts | 41 |
1H chemical shifts | 41 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | wt NS4A N-terminal domain of DENV | 1 |
Entity 1, wt NS4A N-terminal domain of DENV 48 residues - 5226.9633 Da.
1 | SER | LEU | THR | LEU | ASN | LEU | ILE | THR | GLU | MET | ||||
2 | GLY | ARG | LEU | PRO | THR | PHE | MET | THR | GLN | LYS | ||||
3 | ALA | ARG | ASP | ALA | LEU | ASP | ASN | LEU | ALA | VAL | ||||
4 | LEU | HIS | THR | ALA | GLU | ALA | GLY | GLY | ARG | ALA | ||||
5 | TYR | ASN | HIS | ALA | LEU | SER | GLU | LEU |
NMR_sample: wtNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM
NMR_sample_ref: wtNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; DSS 10 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM
Set_1: ionic strength: 200 mM; pH: 6.8; pressure: 1.000 atm; temperature: 303.0 K
Set_2: ionic strength: 200 mM; pH: 6.8; pressure: 1.000 atm; temperature: 303.0 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCA | NMR_sample | isotropic | Set_1 |
2D 1H-15N HSQC/HMQC | NMR_sample | isotropic | Set_1 |
3D HNCO | NMR_sample | isotropic | Set_1 |
HNcaCO (H[N[ca[CO]]]) | NMR_sample | isotropic | Set_1 |
2D 1H-13C HSQC/HMQC | NMR_sample_ref | isotropic | Set_2 |
2D 1H-15N HSQC/HMQC | NMR_sample_ref | isotropic | Set_2 |
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment
BMRB | 25179 |
DBJ | BAD42415 BAD42416 BAD42417 BAH86603 BAH86604 |
EMBL | CAD31751 CAI92123 CAR65133 CAR65134 CAR65135 |
GB | AAA42941 AAA42942 AAA42962 AAA73185 AAA73186 |
REF | NP_056776 NP_739588 |
SP | P07564 P12823 P14337 P14340 P29990 |
AlphaFold | P07564 P12823 P14337 P14340 P29990 |
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