BMRB Entry 25021

Title:
Backbone chemical shifts of murine Roquin-1 ROQ domain (147-326), apo form
Deposition date:
2014-06-13
Original release date:
2014-07-01
Authors:
Schlundt, Andreas; Sattler, Michael
Citation:

Citation: Schlundt, Andreas; Heinz, Gitta; Janowski, Robert; Geerlof, Arie; Stehle, Ralf; Heissmeyer, Vigo; Niessing, Dierk; Sattler, Michael. "Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation."  Nat. Struct. Mol. Biol. 21, 671-678 (2014).
PubMed: 25026077

Assembly members:

Assembly members:
ROQ_domain_of_Roquin-1, polymer, 183 residues, 20758.8 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETTrx1a

Data sets:
Data typeCount
1H chemical shifts148
13C chemical shifts439
15N chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ROQ1

Entities:

Entity 1, ROQ 183 residues - 20758.8 Da.

starts with 147

1   GLYALAMETGLUGLUGLUGLYARGILEARG
2   ALAMETARGALAALAARGSERLEUGLYGLU
3   ARGTHRVALTHRGLULEUILELEUGLNHIS
4   GLNASNPROGLNGLNLEUSERSERASNLEU
5   TRPALAALAVALARGALAARGGLYCYSGLN
6   PHELEUGLYPROALAMETGLNGLUGLUALA
7   LEULYSLEUVALLEULEUALALEUGLUASP
8   GLYSERALALEUSERARGLYSVALLEUVAL
9   LEUPHEVALVALGLNARGLEUGLUPROARG
10   PHEPROGLNALASERLYSTHRSERILEGLY
11   HISVALVALGLNLEULEUTYRARGALASER
12   CYSPHELYSVALTHRLYSARGASPGLUASP
13   SERSERLEUMETGLNLEULYSGLUGLUPHE
14   ARGTHRTYRGLUALALEUARGARGGLUHIS
15   ASPSERGLNILEVALGLNILEALAMETGLU
16   ALAGLYLEUARGILEALAPROASPGLNTRP
17   SERSERLEULEUTYRGLYASPGLNSERHIS
18   LYSSERHISMETGLNSERILEILEASPLYS
19   LEUGLNTHR

Samples:

sample_1: ROQ domain of Roquin-1, [U-98% 13C; U-98% 15N], 200 – 350 uM; TRIS, pH 7.0 20 mM; TCEP 2 mM; sodium chloride 150 mM

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 150 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CCPN_Analysis v2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP Q4VGL6
BMRB 19996
PDB
DBJ BAC23121 BAD32613 BAE24092 BAG53813
EMBL CAG07670 CDQ65000 CDQ67780 CDQ69323
GB AAH68669 AAI36785 AAI38664 AAI44409 AAI52932
REF NP_001020123 NP_001076328 NP_001084548 NP_001108155 NP_001287779
SP Q4VGL6 Q5TC82 Q6NUC6
TPG DAA21174
AlphaFold Q69Z31 Q5TC82 Q4VGL6 Q6NUC6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks