BMRB Entry 2410

Title:
Sequence-specific 1H, 15N, and 13C assignment of the N-terminal domain of the human oncoprotein MDM2 that binds to p53
Deposition date:
2000-04-30
Original release date:
1995-07-31
Authors:
Stoll, Raphael; Renner, Christian; Muhlhahn, Peter; Hansen, Silke; Schumacher, Ralf; Hesse, Friederike; Kaluza, Brigitte; Engh, Richard; Voelter, Wolfgang; Holak, Tad
Citation:

Citation: Stoll, Raphael; Renner, Christian; Muhlhahn, Peter; Hansen, Silke; Schumacher, Ralf; Hesse, Friederike; Kaluza, Brigitte; Engh, Richard; Voelter, Wolfgang; Holak, Tad. "Letter to the Editor: Sequence-specific 1H, 15N, and 13C assignment of the N-terminal domain of the human oncoprotein MDM2 that binds to p53"  J. Biomol. NMR 17, 91-92 (2000).

Assembly members:

Assembly members:
N-terminal domain of the human murine double minute clone 2 protein, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal domain of the human murine double minute clone 2 protein: MCNTNMSVPTDGAVTTSQIP ASEQETLVRPKPLLLKLLKS VGAQKDTYTMKEVLFYLGQY IMTKRLYDEKQQHIVYCSND LLGDLFGVPSFSVKEHRKIY TMIYRNLVVVNQQESSDSS

Data sets:
Data typeCount
13C chemical shifts150
15N chemical shifts83
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain of human MDM21

Entities:

Entity 1, N-terminal domain of human MDM2 119 residues - Formula weight is not available

1   METCYSASNTHRASNMETSERVALPROTHR
2   ASPGLYALAVALTHRTHRSERGLNILEPRO
3   ALASERGLUGLNGLUTHRLEUVALARGPRO
4   LYSPROLEULEULEULYSLEULEULYSSER
5   VALGLYALAGLNLYSASPTHRTYRTHRMET
6   LYSGLUVALLEUPHETYRLEUGLYGLNTYR
7   ILEMETTHRLYSARGLEUTYRASPGLULYS
8   GLNGLNHISILEVALTYRCYSSERASNASP
9   LEULEUGLYASPLEUPHEGLYVALPROSER
10   PHESERVALLYSGLUHISARGLYSILETYR
11   THRMETILETYRARGASNLEUVALVALVAL
12   ASNGLNGLNGLUSERSERASPSERSER

Samples:

sample_1: N-terminal domain of the human murine double minute clone 2 protein, [U-15N], 0.1 – 0.5 mM

sample_2: N-terminal domain of the human murine double minute clone 2 protein, [U-15N; U-13C], 0.1 – 0.5 mM

Ex-cond_1: pH: 7.4; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 11569 18755 18876
PDB
DBJ BAF83030 BAJ17752
EMBL CAA78055 CAD23251 CAD36959 CAD79457 CAH89564
GB AAA60568 AAI48523 AAI52385 AAI52391 AAI53118
PRF 1814460A
REF NP_001124685 NP_001138809 NP_001138811 NP_001253331 NP_002383
SP Q00987
AlphaFold Q00987

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks