BMRB Entry 19591

Title:
Aliphatic 13C Chemical Shift Assignments For the Intermediate State of the FF Domain L24A Mutant
Deposition date:
2013-10-30
Original release date:
2014-02-11
Authors:
Bouvignies, Guillaume; Vallurupalli, Pramodh; Kay, Lewis
Citation:

Citation: Bouvignies, Guillaume; Vallurupalli, Pramodh; Kay, Lewis. "Visualizing side chains of invisible protein conformers by solution NMR."  J. Mol. Biol. 426, 763-774 (2014).
PubMed: 24211467

Assembly members:

Assembly members:
FF_domain_L24A_mutant, polymer, 71 residues, 8194.3 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts203

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FF domain L24A mutant, 11
2FF domain L24A mutant, 21

Entities:

Entity 1, FF domain L24A mutant, 1 71 residues - 8194.3 Da.

1   GLYSERGLNPROALALYSLYSTHRTYRTHR
2   TRPASNTHRLYSGLUGLUALALYSGLNALA
3   PHELYSGLUALALEULYSGLULYSARGVAL
4   PROSERASNALASERTRPGLUGLNALAMET
5   LYSMETILEILEASNASPPROARGTYRSER
6   ALALEUALALYSLEUSERGLULYSLYSGLN
7   ALAPHEASNALATYRLYSVALGLNTHRGLU
8   LYS

Samples:

sample_1: FF domain L24A mutant, [U-100% 13C; U-100% 15N], 2.0 mM; sodium acetate 50 mM; EDTA 0.5 mM; sodium azide 0.02%; sodium chloride 100 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 5.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D aliphatic 13C CESTsample_1isotropicsample_conditions_1

Software:

NMRPipe v7.5 Rev 2012.204.11.07, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17489 18010 26520
PDB
DBJ BAB15016 BAG60744
GB AAC27501 AAC27506 AAD39463 AAH11788 AAH29414
REF NP_001099950 NP_001231502 NP_060362 NP_061255 XP_001365479
SP O75400 Q9R1C7
TPG DAA32724
AlphaFold Q9R1C7 O75400