BMRB Entry 18754

Title:
Chemical shift of E coli. IscU Prolyl residues in D-states
Deposition date:
2012-10-01
Original release date:
2013-02-12
Authors:
Dai, Ziqi; Tonelli, Marco; Markley, John
Citation:

Citation: Dai, Ziqi; Tonelli, Marco; Markley, John. "Metamorphic Protein IscU Changes Conformation by cis-trans Isomerizations of Two Peptidyl-Prolyl Peptide Bonds."  Biochemistry 51, 9595-9602 (2012).
PubMed: 23110687

Assembly members:

Assembly members:
IscU, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrc99A

Data sets:
Data typeCount
13C chemical shifts20
15N chemical shifts4
1H chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1D-state1

Entities:

Entity 1, D-state 128 residues - Formula weight is not available

1   METALATYRSERGLULYSVALILEASPHIS
2   TYRGLUASNPROARGASNVALGLYSERPHE
3   ASPASNASNASPGLUASNVALGLYSERGLY
4   METVALGLYALAPROALACYSGLYASPVAL
5   METLYSLEUGLNILELYSVALASNASPGLU
6   GLYILEILEGLUASPALAARGPHELYSTHR
7   TYRGLYCYSGLYSERALAILEALASERSER
8   SERLEUVALTHRGLUTRPVALLYSGLYLYS
9   SERLEUASPGLUALAGLNALAILELYSASN
10   THRASPILEALAGLUGLULEUGLULEUPRO
11   PROVALLYSILEHISCYSSERILELEUALA
12   GLUASPALAILELYSALAALAILEALAASP
13   TYRLYSSERLYSARGGLUALALYS

Samples:

13C-15N-Pro: IscU, [U-13C; U-15N]-Pro, 1 – 5 mM; D2O 10%; DSS 50 uM; EDTA 0.5 mM; DTT 5 mM; sodium azide 50 uM

45C: pH: 8; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC13C-15N-Proisotropic45C
3D HCCH-TOCSY13C-15N-Proisotropic45C
3D 1H-13C NOESY aliphatic13C-15N-Proisotropic45C

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 15967 16245 16603 17282 17836 17837 17844 18359 18360 18361 18362 18381 18750
PDB
DBJ BAA16423 BAB36818 BAG78339 BAH64655 BAI26774
EMBL CAD02745 CAP76981 CAQ32902 CAQ88187 CAQ99420
GB AAC75582 AAG57643 AAL21436 AAN44075 AAN81505
PIR AE0824
REF NP_311422 NP_417024 NP_457073 NP_461477 NP_708368
SP P0ACD4 P0ACD5 P0ACD6 P0ACD7
AlphaFold P0ACD4 P0ACD5 P0ACD6 P0ACD7