BMRB Entry 17769

Title:
UBIQUITIN NMR STRUCTURE
Deposition date:
2011-07-07
Original release date:
2011-08-16
Authors:
Cornilescu, G.; Marquardt, J.; Ottiger, M.; Bax, A.
Citation:

Citation: Cornilescu, G.; Marquardt, J.; Ottiger, M.; Bax, A.. "Validation of Protein Structure from Anisotropic Carbonyl Chemical Shifts in a Dilute Liquid Crystalline Phase"  J. Am. Chem. Soc. 120, 6836-6837 (1998).

Assembly members:

Assembly members:
Ubiquitin, polymer, 76 residues, 8576.914 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts348
15N chemical shifts83
1H chemical shifts570

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PROTEIN (UBIQUITIN)1

Entities:

Entity 1, PROTEIN (UBIQUITIN) 76 residues - 8576.914 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample: PROTEIN (UBIQUITIN), [U-99% 13C; U-99% 15N], mM; Phosphate Buffer 50 mM; D2O 7%; H2O 93%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-SEPARATED_NOESYsampleisotropicsample_conditions_1
4D_13C-SEPARATED_NOESYsampleisotropicsample_conditions_1
4D_13C/15N-SEPARATED_NOESYsampleisotropicsample_conditions_1
MOREsampleisotropicsample_conditions_1

Software:

X-PLOR v3.840, BRUNGER ET AL. - refinement, structure solution

NMRPipe v1.70, DELAGLIO ET AL. - processing

NMR spectrometers:

  • BRUKER DMX 600 MHz

Related Database Links:

BMRB 11505 11547 15047 15410 15689 15907 16228 16582 16626 16895 17181 17439 17919 18582 18583 18584 18610 18611 18737 19406 19412 25070 25123 25601 26604 4245 4375
PDB
DBJ BAA03983 BAA09860 BAA11842 BAA11843 BAA23486
EMBL CAA25706 CAA26488 CAA28495 CAA30183 CAA30815
GB AAA02769 AAA28154 AAA28997 AAA28998 AAA28999
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1101405A 1212243A 1212243B 1212243C
REF NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPD FAA00319
TPE CEL68433 CEL70397 CEL75964 CEL78064
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295
AlphaFold P0CG47 P0CG48 P0C276 P0C275 P0C273

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks