BMRB Entry 16459

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16459

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Title:
Solution Structure of the Ran-binding Domain 2 of RanBP and its Interaction with the C Terminus of Ran
Deposition date:
2009-08-19
Original release date:
2010-03-08
Authors:
Geyer, J.; Doker, Rolf; Kremer, Werner; Zhao, Xiaodong; Kuhlmann, Jurgen; Kalbitzer, Hans
Citation:

Citation: Geyer, J.; Doker, Rolf; Kremer, Werner; Zhao, Xiaodong; Kuhlmann, Jurgen; Kalbitzer, Hans. "Solution Structure of the Ran-binding Domain 2 of RanBP2 and its Interaction with the C Terminus of Ran"  J. Mol. Biol. 348, 711-725 (2005).
PubMed: 15826666

Assembly members:

Assembly members:
RanBD2(delta)N, polymer, 130 residues, Formula weight is not available
peptide_2, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis   Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RanBD2(delta)N: GSGEEDEKVLYSQRVKLFRF DAEVSQWKERGLGNLKILKN EVNGKLRMLMRREQVLKVCA NHWITTTMNLKPLSGSDRAW MWLASDFSDGDAKLEQLAAK FKTPELAEEFKQKFEECQRL LLDIPLQTPK
peptide_2: MDPALAAQYEHDLEVAQTTA LPDEDDDL

Data sets:
  • binding_constants
Data typeCount
binding constants2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RanBD2(delta)N1
2Ran C2

Entities:

Entity 1, RanBD2(delta)N 130 residues - Formula weight is not available

1   GLYSERGLYGLUGLUASPGLULYSVALLEU
2   TYRSERGLNARGVALLYSLEUPHEARGPHE
3   ASPALAGLUVALSERGLNTRPLYSGLUARG
4   GLYLEUGLYASNLEULYSILELEULYSASN
5   GLUVALASNGLYLYSLEUARGMETLEUMET
6   ARGARGGLUGLNVALLEULYSVALCYSALA
7   ASNHISTRPILETHRTHRTHRMETASNLEU
8   LYSPROLEUSERGLYSERASPARGALATRP
9   METTRPLEUALASERASPPHESERASPGLY
10   ASPALALYSLEUGLUGLNLEUALAALALYS
11   PHELYSTHRPROGLULEUALAGLUGLUPHE
12   LYSGLNLYSPHEGLUGLUCYSGLNARGLEU
13   LEULEUASPILEPROLEUGLNTHRPROLYS

Entity 2, Ran C 28 residues - Formula weight is not available

1   METASPPROALALEUALAALAGLNTYRGLU
2   HISASPLEUGLUVALALAGLNTHRTHRALA
3   LEUPROASPGLUASPASPASPLEU

Samples:

sample_1: RanBD2(delta)N 0.5 mM; peptide 20.5 – 2.5 mM; sodium sulfate 150 mM; DTE 10 mM; EDTA 0.5 mM; sodium azide 1 mM; DSS 0.1 mM; potassium phosphate buffer 10 mM; D2O 8%; H2O 92%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

AUREMOL, Bruker Biospin - data analysis

TALOS, Cornilescu, Delaglio and Bax - structure solution

X-PLOR NIH v2.9.6, Schwieters, Kuszewski, Tjandra and Clore - refinement

Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Tho - data analysis

Molmol, Koradi, Billeter and Wuthrich - visualization

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
BMRB 19852 19854
DBJ BAB27034 BAB27105 BAB93486 BAC36040 BAC40068
EMBL CAA77980 CAG29343 CAH92646 CAH93110 CAI29709
GB AAA36546 AAA64247 AAB20486 AAB24940 AAB26214
REF NP_001029877 NP_001126549 NP_001129282 NP_001155348 NP_001181913
SP P62825 P62826 P62827 P62828 Q3T054
TPG DAA20885
AlphaFold P62827 P62828 Q3T054 P62826 P62825