BMRB Entry 15650

Title:
Solution structure of CaM complexed to DRP1p
Deposition date:
2008-02-04
Original release date:
2009-10-20
Authors:
Bertini, Ivano; Luchinat, Claudio; Parigi, Giacomo; Yuan, Jing
Citation:

Citation: Bertini, Ivano; Kursula, Petri; Luchinat, Claudio; Parigi, Giacomo; Vahokoski, Juha; Yuan, Jing. "Accurate solution structures of proteins from X-ray data and a minimal set of NMR data: calmodulin-peptide complexes as examples"  J. Am. Chem. Soc. 131, 5134-5144 (2009).
PubMed: 19317469

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, 16650.387 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b-CaM

Data sets:
Data typeCount
13C chemical shifts419
15N chemical shifts145
1H chemical shifts145
T1 relaxation values110
T2 relaxation values110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1
2Calcium ions, 12
3Calcium ions, 22
4Calcium ions, 32
5Calcium ions, 42

Entities:

Entity 1, Calmodulin 148 residues - 16650.387 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASP
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, Calcium ions, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: calmodulin, [U-100% 13C; U-100% 15N], 0.4 mM; Hepes 30 mM; NaCl 150 mM; TCEP 0.5 mM

sample_2: calmodulin, [U-100% 15N], 0.4 mM; Hepes 30 mM; NaCl 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
IPAPsample_2isotropicsample_conditions_1
15N relaxation measurement R1sample_2isotropicsample_conditions_1
15N relaxation measurement R2sample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA v2.0, Keller and Wuthrich - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15852 16418 16465 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0608335A 0711223A
REF NP_001008160 NP_001009759 NP_001039714 NP_001040234 NP_001080864
SP O16305 P02594 P02595 P11121 P21251
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988
AlphaFold P11121 P21251 O16305 P02594 P02595

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks