BMRB Entry 15594

Title:
Rapid Measurement of Pseudocontact Shifts in Paramagnetic Proteins by GFT NMR Spectroscopy
Deposition date:
2007-12-13
Original release date:
2008-02-11
Authors:
Barnwal, Ravi Pratap; Rout, Ashok K; Chary, Kandala VR; Atreya, HS
Citation:

Citation: Barnwal, Ravi Pratap; Rout, Ashok K; Chary, Kandala VR; Atreya, HS. "Rapid Measurement of Pseudocontact Shifts in Paramagnetic Proteins by GFT NMR Spectroscopy"  Open Mag. Res. J. 1, 16-28 (2008).

Assembly members:

Assembly members:
calbindin_in_presence_of_Yb3+, polymer, 75 residues, Formula weight is not available
CA, non-polymer, Formula weight is not available
YB, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: NA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calbindin_in_presence_of_Yb3+: KSPEELKGIFEKYAAKEGDP NQLSKEELKLLLQTEFPSLL KGMSTLDELFEELDKNGDGE VSFEEFQVLVKKISQ

Data sets:
Data typeCount
13C chemical shifts113
15N chemical shifts52
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sub unit 11
2CA2
3YB3

Entities:

Entity 1, sub unit 1 75 residues - Formula weight is not available

1   LYSSERPROGLUGLULEULYSGLYILEPHE
2   GLULYSTYRALAALALYSGLUGLYASPPRO
3   ASNGLNLEUSERLYSGLUGLULEULYSLEU
4   LEULEUGLNTHRGLUPHEPROSERLEULEU
5   LYSGLYMETSERTHRLEUASPGLULEUPHE
6   GLUGLULEUASPLYSASNGLYASPGLYGLU
7   VALSERPHEGLUGLUPHEGLNVALLEUVAL
8   LYSLYSILESERGLN

Entity 2, CA - Formula weight is not available

1   CA

Entity 3, YB - Formula weight is not available

1   YB

Samples:

sample_1: sub unit 1, [U-98% 13C; U-98% 15N], 1.6 mM; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
GFT HNCOsample_1isotropicsample_conditions_1
GFT HN(CO)CAsample_1isotropicsample_conditions_1
GFT CB(CACO)NHsample_1isotropicsample_conditions_1
GFT HNHAsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16340 16758 247 325 326 327 4581
PDB
GB AAA30420 AAA72542 AAI18481 ADO85633
PRF 0707237A:PDB=3ICB
REF NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP P02633
TPG DAA12577
AlphaFold P02633

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks