BMRB Entry 15589

Title:
Backbone H, N and CA Chemical Shift Assignments for ApoMb(1-77) in Complex with DnaK Binding Domain
Deposition date:
2007-12-11
Original release date:
2008-06-30
Authors:
Kurt, Nese; Cavagnero, Silvia
Citation:

Citation: Kurt, Nese; Cavagnero, Silvia. "Nonnative helical motif in a chaperone-bound protein fragment"  Biophys. J. 94, 48-50 (2008).
PubMed: 18192369

Assembly members:

Assembly members:
Apomyoglobin_(1-77), polymer, 77 residues, Formula weight is not available
DnaK-beta, polymer, . residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Physeter catodon   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts47
15N chemical shifts46
1H chemical shifts46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Substrate1
2Chaperone2

Entities:

Entity 1, Substrate 77 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSHISGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYS

Entity 2, Chaperone - Formula weight is not available

Samples:

sample_1: Apomyoglobin (1-77), [U-99% 13C; U-99% 15N], 100 uM; DnaK-beta, [U-2H], 100 uM; sodium acetate 10 mM; D2O, [U-100% 2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 10 mM; pH: 5.8; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676 4695
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185
AlphaFold P02185

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks