BMRB Entry 15237

Title:
1H, 15N chemical shift backbone assignment of the D31K mutant of the protein CyaY
Deposition date:
2007-05-04
Original release date:
2007-05-18
Authors:
Pastore, Chiara; Pastore, Annalisa; Correia, Ana; Gomes, Claudio
Citation:

Citation: Adinolfi, Salvatore; Iannuzzi, Clara; Prischi, Filippo; Pastore, Chiara; Iametti, Stefania; Martin, Stephen; Bonomi, Franco; Pastore, Annalisa. "Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS."  Nat. Struct. Mol. Biol. 16, 390-396 (2009).
PubMed: 19305405

Assembly members:

Assembly members:
CyaYD31K, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CyaYD31K1

Entities:

Entity 1, CyaYD31K 108 residues - Formula weight is not available

1   GLYALAMETASNASPSERGLUPHEHISARG
2   LEUALAASPGLNLEUTRPLEUTHRILEGLU
3   GLUARGLEUASPASPTRPASPGLYASPSER
4   ASPILELYSCYSGLUILEASNGLYGLYVAL
5   LEUTHRILETHRPHEGLUASNGLYSERLYS
6   ILEILEILEASNARGGLNGLUPROLEUHIS
7   GLNVALTRPLEUALATHRLYSGLNGLYGLY
8   TYRHISPHEASPLEULYSGLYASPGLUTRP
9   ILECYSASPARGSERGLYGLUTHRPHETRP
10   ASPLEULEUGLUGLNALAALATHRGLNGLN
11   ALAGLYGLUTHRVALSERPHEARG

Samples:

sample_1: CyaYD31K, [U-98% 15N], 0.5 mM; NaP 20 mM; NaCl 50 mM; DTT 2 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15236 15244
PDB
DBJ BAB38160 BAE77494 BAG79612 BAI27941 BAI33064
EMBL CAA47281 CAP78250 CAQ34148 CAQ91159 CAR00776
GB AAA67603 AAC76810 AAG58999 AAN45316 AAN83159
REF NP_312764 NP_418251 NP_709609 WP_000999934 WP_000999935
SP A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7
AlphaFold A7ZU09 B1LLX7 B1IW97 A1AHX3 A8A6R1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks