BMRB Entry 15078

Title:
Dynein Light Chain LC8 at pH 5.5, complex with IC peptide
Deposition date:
2006-12-14
Original release date:
2007-10-17
Authors:
Benison, Gregory
Citation:

Citation: Benison, Gregory; Karplus, P.; Barbar, Elisar. "Structure and dynamics of LC8 complexes with KXTQT-motif peptides: swallow and dynein intermediate chain compete for a common site"  J. Mol. Biol. 371, 457-468 (2007).
PubMed: 17570393

Assembly members:

Assembly members:
LC8, polymer, 89 residues, Formula weight is not available
IC, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-15da

Data sets:
Data typeCount
13C chemical shifts154
15N chemical shifts85
1H chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC81
2ic2

Entities:

Entity 1, LC8 89 residues - Formula weight is not available

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Entity 2, ic 16 residues - Formula weight is not available

Dynein intermediate chain IC74 residues 123-138

1   LYSGLUTHRLEUVALTYRTHRLYSGLNTHR
2   GLNTHRTHRSERTHRGLY

Samples:

sample_1: LC8, [U-100% 13C; U-100% 15N], 1 mM; IC 50 mM; potassium phosphate 50 mM; sodium citrate 50 mM; glycerol 3%; DSS 1 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15076 15077 15953 16847 17692 15441
PDB
EMBL CDQ62741 CDQ75050
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975 AAC70942 AAC78307 EDX18442
REF NP_001135068 NP_001177683 NP_001188181 NP_001245530 NP_001245836 XP_002107459
SP O96860 Q24117
TPG DAA34424
AlphaFold O96860 Q24117

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks