BMRB Entry 4798

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Frq1
Published: 2000-09-03

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PDB ID: 1fpw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4798
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Frq1

Deposition date:

2000-07-31

Original release date:

2000-09-03

Authors:

Ames, James; Hendricks, Kristin; Strahl, Thomas; Huttner, Inken; Hamasaki, Nobuko; Thorner, Jeremy

Citation:

Citation: Ames, James; Hendricks, Kristin; Strahl, Thomas; Huttner, Inken; Hamasaki, Nobuko; Thorner, Jeremy. "Structure and Calcium-binding Properties of frq1, a Novel Calcium Sensor in the Yeast Saccharomyces cerevisiae" Biochemistry 39, 12149-12161 (2000).

Assembly members:

Assembly members:
Yeast frequenin, polymer, 190 residues, 22000 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: budding yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yeast frequenin: MGAKTSKLSKDDLTCLKQST YFDRREIQQWHKGFLRDCPS GQLAREDFVKIYKQFFPFGS PEDFANHLFTVFDKDNNGFI HFEEFITVLSTTSRGTLEEK LSWAFELYDLNHDGYITFDE MLTIVASVYKMMGSMVTLNE DEATPEMRVKKIFKLMDKNE DGYITLDEFREGSKVDPSII GALNLYDGLI

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	393
13C chemical shifts	355
15N chemical shifts	114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Frq1	1
2	Ca I	2
3	Ca II	2
4	Ca III	2

Entities:

Entity 1, Frq1 190 residues - 22000 Da.

1	MET	GLY	ALA	LYS	THR	SER	LYS	LEU	SER	LYS
2	ASP	ASP	LEU	THR	CYS	LEU	LYS	GLN	SER	THR
3	TYR	PHE	ASP	ARG	ARG	GLU	ILE	GLN	GLN	TRP
4	HIS	LYS	GLY	PHE	LEU	ARG	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ALA	ARG	GLU	ASP	PHE	VAL	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	SER
7	PRO	GLU	ASP	PHE	ALA	ASN	HIS	LEU	PHE	THR
8	VAL	PHE	ASP	LYS	ASP	ASN	ASN	GLY	PHE	ILE
9	HIS	PHE	GLU	GLU	PHE	ILE	THR	VAL	LEU	SER
10	THR	THR	SER	ARG	GLY	THR	LEU	GLU	GLU	LYS
11	LEU	SER	TRP	ALA	PHE	GLU	LEU	TYR	ASP	LEU
12	ASN	HIS	ASP	GLY	TYR	ILE	THR	PHE	ASP	GLU
13	MET	LEU	THR	ILE	VAL	ALA	SER	VAL	TYR	LYS
14	MET	MET	GLY	SER	MET	VAL	THR	LEU	ASN	GLU
15	ASP	GLU	ALA	THR	PRO	GLU	MET	ARG	VAL	LYS
16	LYS	ILE	PHE	LYS	LEU	MET	ASP	LYS	ASN	GLU
17	ASP	GLY	TYR	ILE	THR	LEU	ASP	GLU	PHE	ARG
18	GLU	GLY	SER	LYS	VAL	ASP	PRO	SER	ILE	ILE
19	GLY	ALA	LEU	ASN	LEU	TYR	ASP	GLY	LEU	ILE

Entity 2, Ca I - Ca - 40.078 Da.

1

CA

Samples:

sample_1: Yeast frequenin, [U-95% 13C; U-90% 15N], 0.8 mM; octyl glucoside 20 mM

Ex-cond_1: pH: 6.6; temperature: 318 K; ionic strength: 0.05 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	sample_1	not available	Ex-cond_1
1H-15N TOCSY	sample_1	not available	Ex-cond_1
HNCO	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1
CBCACONH	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz

PDB	1FPW 2JU0
DBJ	GAA22591
EMBL	CAY78874
GB	AAB64809 AHY75328 EDN60701 EDV07959 EDZ72897
REF	NP_010661 XP_011104984
SP	Q06389
TPG	DAA12214
AlphaFold	Q06389

1	MET	GLY	ALA	LYS	THR	SER	LYS	LEU	SER	LYS
2	ASP	ASP	LEU	THR	CYS	LEU	LYS	GLN	SER	THR
3	TYR	PHE	ASP	ARG	ARG	GLU	ILE	GLN	GLN	TRP
4	HIS	LYS	GLY	PHE	LEU	ARG	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ALA	ARG	GLU	ASP	PHE	VAL	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	SER
7	PRO	GLU	ASP	PHE	ALA	ASN	HIS	LEU	PHE	THR
8	VAL	PHE	ASP	LYS	ASP	ASN	ASN	GLY	PHE	ILE
9	HIS	PHE	GLU	GLU	PHE	ILE	THR	VAL	LEU	SER
10	THR	THR	SER	ARG	GLY	THR	LEU	GLU	GLU	LYS
11	LEU	SER	TRP	ALA	PHE	GLU	LEU	TYR	ASP	LEU
12	ASN	HIS	ASP	GLY	TYR	ILE	THR	PHE	ASP	GLU
13	MET	LEU	THR	ILE	VAL	ALA	SER	VAL	TYR	LYS
14	MET	MET	GLY	SER	MET	VAL	THR	LEU	ASN	GLU
15	ASP	GLU	ALA	THR	PRO	GLU	MET	ARG	VAL	LYS
16	LYS	ILE	PHE	LYS	LEU	MET	ASP	LYS	ASN	GLU
17	ASP	GLY	TYR	ILE	THR	LEU	ASP	GLU	PHE	ARG
18	GLU	GLY	SER	LYS	VAL	ASP	PRO	SER	ILE	ILE
19	GLY	ALA	LEU	ASN	LEU	TYR	ASP	GLY	LEU	ILE

1	MET	GLY	ALA	LYS	THR	SER	LYS	LEU	SER	LYS
2	ASP	ASP	LEU	THR	CYS	LEU	LYS	GLN	SER	THR
3	TYR	PHE	ASP	ARG	ARG	GLU	ILE	GLN	GLN	TRP
4	HIS	LYS	GLY	PHE	LEU	ARG	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ALA	ARG	GLU	ASP	PHE	VAL	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	SER
7	PRO	GLU	ASP	PHE	ALA	ASN	HIS	LEU	PHE	THR
8	VAL	PHE	ASP	LYS	ASP	ASN	ASN	GLY	PHE	ILE
9	HIS	PHE	GLU	GLU	PHE	ILE	THR	VAL	LEU	SER
10	THR	THR	SER	ARG	GLY	THR	LEU	GLU	GLU	LYS
11	LEU	SER	TRP	ALA	PHE	GLU	LEU	TYR	ASP	LEU
12	ASN	HIS	ASP	GLY	TYR	ILE	THR	PHE	ASP	GLU
13	MET	LEU	THR	ILE	VAL	ALA	SER	VAL	TYR	LYS
14	MET	MET	GLY	SER	MET	VAL	THR	LEU	ASN	GLU
15	ASP	GLU	ALA	THR	PRO	GLU	MET	ARG	VAL	LYS
16	LYS	ILE	PHE	LYS	LEU	MET	ASP	LYS	ASN	GLU
17	ASP	GLY	TYR	ILE	THR	LEU	ASP	GLU	PHE	ARG
18	GLU	GLY	SER	LYS	VAL	ASP	PRO	SER	ILE	ILE
19	GLY	ALA	LEU	ASN	LEU	TYR	ASP	GLY	LEU	ILE