BMRB Entry 4380

Title:
1H, 15N and 13C Resonance Assignments and Monomeric Structure of the Amino-Terminal Extracellular Domain of Epithelial Cadherin
Deposition date:
1999-06-30
Original release date:
2007-07-13
Authors:
Overduin, Michael; Tong, Kit; Kay, Cyril; Ikura, Mitsuhiko
Citation:

Citation: Overduin, Michael; Tong, Kit; Kay, Cyril; Ikura, Mitsuhiko. "1H, 15N and 13C Resonance Assignments and Monomeric Structure of the Amino-Terminal Extracellular Domain of Epithelial Cadheri"  J. Biomol. NMR 7, 173-189 (1996).

Assembly members:

Assembly members:
Epithelial cadherin, polymer, 146 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus Musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts133
1H chemical shifts792

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E-cadherin domain 11

Entities:

Entity 1, E-cadherin domain 1 146 residues - Formula weight is not available

1   METARGASPTRPVALILEPROPROILESER
2   CYSPROGLUASNGLULYSGLYGLUPHEPRO
3   LYSASNLEUVALGLNILELYSSERASNARG
4   ASPLYSGLUTHRLYSVALPHETYRSERILE
5   THRGLYGLNGLYALAASPLYSPROPROVAL
6   GLYVALPHEILEILEGLUARGGLUTHRGLY
7   TRPLEULYSVALTHRGLNPROLEUASPARG
8   GLUALAILEALALYSTYRILELEUTYRSER
9   HISALAVALSERSERASNGLYGLUALAVAL
10   GLUASPPROMETGLUILEVALILETHRVAL
11   THRASPGLNASNASPASNARGPROGLUPHE
12   THRGLNGLUVALPHEGLUGLYSERVALALA
13   GLUGLYALAVALPROGLYTHRSERVALMET
14   LYSVALSERALATHRASPALAASPASPASP
15   VALASNTHRTYRASNALA

Samples:

sample_1: Epithelial cadherin, [U-13C; U-15N], 0.1 – 2 mM; KCl 100 mM; perdeuterated dithiothreitol 10 mM; perdeuterated Tris 20 mM; sodium azide 50 uM; CaCl2 10 mM

condition_1: pH*: 7.2; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecondition_1

Software:

PIPP - spectral analysis

NMR spectrometers:

  • Varian UNITY-plus 500 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks