BMRB Entry 7384

Name: Solution Structure of Human Immunodificiency Virus Type-2 Nucleocapsid Protein
Published: 2008-08-14

Click here to enlarge.

PDB ID: 2ec7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7384
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure of Human Immunodificiency Virus Type-2 Nucleocapsid Protein

Deposition date:

2007-03-21

Original release date:

2008-08-14

Authors:

Matsui, T.; Kodera, Y.; Tanaka, T.; Endoh, H.; Tanaka, H.; Miyauchi, E.; Komatsu, H.; Kohno, T.; Maeda, T.

Citation:

Citation: Matsui, Takashi; Kodera, Yoshio; Miyauchi, Emi; Tanaka, Hidekazu; Endoh, Hiroshi; Komatsu, Hiroyoshi; Tanaka, Takeshi; Kohno, Toshiyuki; Maeda, Tadakazu. "Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality." Biochem. Biophys. Res. Commun. 358, 673-678 (2007).
PubMed: 17511966

Assembly members:

Assembly members:
Gag_polyprotein_(Pr55Gag), polymer, 49 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: HIV-2 Taxonomy ID: 11709 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus Human immunodeficiency virus 2

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gag_polyprotein_(Pr55Gag): AQQRKVIRCWNCGKEGHSAR QCRAPRRQGCWKCGKTGHVM AKCPERQAG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	163
15N chemical shifts	54
1H chemical shifts	300

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Gag_polyprotein_(Pr55Gag)	1
2	ZINC 1	2
3	ZINC 2	2

Entities:

Entity 1, Gag_polyprotein_(Pr55Gag) 49 residues - 65.409 Da.

1

ALA

GLN

ARG

LYS

VAL

ILE

ARG

CYS

TRP

2

ASN

CYS

GLY

LYS

GLU

GLY

HIS

SER

ALA

ARG

3

GLN

CYS

ARG

ALA

PRO

ARG

GLN

GLY

CYS

4

TRP

LYS

CYS

GLY

LYS

THR

GLY

HIS

VAL

MET

5

ALA

LYS

CYS

PRO

GLU

ARG

GLN

ALA

GLY

Entity 2, ZINC 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Gag_polyprotein_(Pr55Gag), [U-13C; U-15N], 1.5 mM; ZnCl2 3.2 mM; H2O 90%; D2O 10%

sample_2: Gag_polyprotein_(Pr55Gag), [U-13C; U-15N], 1.5 mM; ZnCl2 3.2 mM; D2O 99.96%

sample_conditions_1: ionic strength: . .; pH: 5.8; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_13C-separated_NOESY	sample_1	isotropic	sample_conditions_1
3D_15N-separated_NOESY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_2	isotropic	sample_conditions_1
HNHA	sample_1	isotropic	sample_conditions_1
2D TOCSY	sample_2	isotropic	sample_conditions_1

Software:

ANSIG v3.3, Kraulis, P.J. - collection

AZARA v2.7, Boucher, W. - processing

X-PLOR NIH v2.9.9, PARDI, A. - structure solution

X-PLOR NIH v2.9.9, PARDI, A. - refinement

NMR spectrometers:

Bruker DMX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks