BMRB Entry 7376

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7376
MolProbity Validation Chart

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Title:
Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states
Deposition date:
2007-03-16
Original release date:
2007-08-23
Authors:
Arunkumar, Alphonse; Pennella, Mario; Kong, Xiangming; David, Giedroc
Citation:

Citation: Arunkumar, Alphonse; Pennella, Mario; Kong, Xiangming; Giedroc, David. "Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states"  Biomol. NMR Assignments 1, 99-101 (2007).
PubMed: 19636838

Assembly members:

Assembly members:
CzrA_Chain, polymer, 106 residues, 11988.69 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CzrA_Chain: MAEQYSEINTDTLERVTEIF KALGDYNRIRIMELLSVSEA SVGHISHQLNLSQSNVSHQL KLLKSVHLVKAKRQGQSMIY SLDDIHVATMLKQAIHHANH PKESGL

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts112
1H chemical shifts268

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1czra11
2czra21
3Zn12
4zn22

Entities:

Entity 1, czra1 106 residues - 11988.69 Da.

1   METALAGLUGLNTYRSERGLUILEASNTHR
2   ASPTHRLEUGLUARGVALTHRGLUILEPHE
3   LYSALALEUGLYASPTYRASNARGILEARG
4   ILEMETGLULEULEUSERVALSERGLUALA
5   SERVALGLYHISILESERHISGLNLEUASN
6   LEUSERGLNSERASNVALSERHISGLNLEU
7   LYSLEULEULYSSERVALHISLEUVALLYS
8   ALALYSARGGLNGLYGLNSERMETILETYR
9   SERLEUASPASPILEHISVALALATHRMET
10   LEULYSGLNALAILEHISHISALAASNHIS
11   PROLYSGLUSERGLYLEU

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

Samples:

ZnCzrA_sample_1: CzrA, [U-100% 13C; U-100% 15N], 2 ± 0.1 mM; Zinc ion 2.4 mM; MES, [U-100% 2H], 10 ± 0.5 mM; sodium chloride 50 ± 1 mM

ZnCzrA_sample_2: CzrA, [U-2H; U-15N]; [U-13C]-Ile,Leu,Val-methyl, 2 ± 0.1 mM; Zinc ion 2.4 mM; MES, [U-100% 2H], 10 ± 0.5 mM; sodium chloride 50 ± 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYZnCzrA_sample_1isotropicsample_conditions_1
2D 1H-13C HMQCZnCzrA_sample_1isotropicsample_conditions_1
3D CBCA(CO)NHZnCzrA_sample_1isotropicsample_conditions_1
3D HNCACBZnCzrA_sample_1isotropicsample_conditions_1
3D C(CO)NHZnCzrA_sample_1isotropicsample_conditions_1
3D H(CCO)NHZnCzrA_sample_1isotropicsample_conditions_1
3D HNCOZnCzrA_sample_1isotropicsample_conditions_1
2D 1H-15N IPAP-HSQCZnCzrA_sample_1anisotropicsample_conditions_1
3D 1H-15N NOESY-TROSYZnCzrA_sample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

MARS, (MARS)-Jung and Zweckstetter - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks