BMRB Entry 6760

Name: NMR assignment of the mTOR domain responsible for rapamycin binding
Published: 2006-03-10

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PDB ID: 2npu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6760
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of the mTOR domain responsible for rapamycin binding

Deposition date:

2005-08-04

Original release date:

2006-03-10

Authors:

Veverka, Vaclav; Lennie, Gregor; Crabbe, Tom; Bird, Ian; Taylor, Richard; Carr, Mark

Citation:

Citation: Veverka, Vaclav; Lennie, Gregor; Crabbe, Tom; Bird, Ian; Taylor, Richard; Carr, Mark. "NMR assignment of the mTOR domain responsible for rapamycin binding" J. Biomol. NMR 36, 3-3 (2006).
PubMed: 16456707

Assembly members:

Assembly members:
FRB domain, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FRB domain: MSYYHHHHHHDYDIPTTENL YFQGAMELIRVPILWHEMWH EGLEEASRLYFGERNVKGMF EVLEPLHAMMERGPQTLKET SFNQAYGRDLMEAQEWCRKY MKSGNVKDLTQAWDLYYHVF RRISKQ

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	347
15N chemical shifts	117
1H chemical shifts	803

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FRB domain	1

Entities:

Entity 1, FRB domain 126 residues - Formula weight is not available

1

MET

SER

TYR

HIS

2

ASP

TYR

ASP

ILE

PRO

THR

GLU

ASN

LEU

3

TYR

PHE

GLN

GLY

ALA

MET

GLU

LEU

ILE

ARG

4

VAL

PRO

ILE

LEU

TRP

HIS

GLU

MET

TRP

HIS

5

GLU

GLY

LEU

GLU

ALA

SER

ARG

LEU

TYR

6

PHE

GLY

GLU

ARG

ASN

VAL

LYS

GLY

MET

PHE

7

GLU

VAL

LEU

GLU

PRO

LEU

HIS

ALA

MET

8

GLU

ARG

GLY

PRO

GLN

THR

LEU

LYS

GLU

THR

9

SER

PHE

ASN

GLN

ALA

TYR

GLY

ARG

ASP

LEU

10

MET

GLU

ALA

GLN

GLU

TRP

CYS

ARG

LYS

TYR

11

MET

LYS

SER

GLY

ASN

VAL

LYS

ASP

LEU

THR

12

GLN

ALA

TRP

ASP

LEU

TYR

HIS

VAL

PHE

13

ARG

ILE

SER

LYS

GLN

Samples:

sample_1: FRB domain, [U-15N], 0.1 mM

sample_2: FRB domain, [U-15N; U-13C], 0.1 mM

sample_3: FRB domain 0.1 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC_NOESY	not available	not available	conditions_1
HNCACB	not available	not available	conditions_1
CBCACONH	not available	not available	conditions_1
HCCH-TOCSY	not available	not available	conditions_1
1H13C_HSQC_NOESY	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	11471 16933
PDB	1AUE 1FAP 1NSG 2FAP 2GAQ 2NPU 2RSE 3FAP 4DRH 4DRI 4DRJ 4FAP
REF	XP_007536126 XP_007895970